Ganglionic and arterial release of neuropeptide Y by bullfrog sympathetic neurons

M. Richard Thorne, Susan Smith, John P. Horn

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    Abstract

    Sympathetic C neurons in lumbar paravertebral ganglia of the bullfrog have previously been shown to be vasomotor in function and to express neuropeptide Y (NPY). In the present experiments, a sensitive radioimmunoassay was used to measure the NPY content of ganglia and the descending abdominal aorta and to measure the overflow of NPY evoked by depolarizing concentrations of K+. Paravertebral ganglia 9 and 10 contain 3.1 pg NPY/μg protein and the aorta contains 0.18 pg NPY/μg protein. During 20-min depolarizations in high K+ (58 mM) Ringer, the ganglia released ∼ 5% of their NPY content and the aorta released ∼ 2% of its NPY content. Pretreatment of the tissues with Ringer containing 0.18 mM Ca2+, 8 mM Mg2+, and 1 mM Co2+ blocked the NPY release elicited by high K+. These findings provide further evidence that NPY is a postganglionic co-transmitter in sympathetic C neurons of the bullfrog.

    Original languageEnglish (US)
    Pages (from-to)231-236
    Number of pages6
    JournalJournal of the Autonomic Nervous System
    Volume38
    Issue number3
    DOIs
    StatePublished - May 15 1992

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    Keywords

    • Aorta
    • Bullfrog
    • Neuropeptide Y
    • Radioimmunoassay
    • Sympathetic neuron
    • Vasomotor neuron

    ASJC Scopus subject areas

    • Neuroscience(all)
    • Physiology
    • Clinical Neurology

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