Ganglionic and arterial release of neuropeptide Y by bullfrog sympathetic neurons

M. Richard Thorne, M (Susan) Smith, John P. Horn

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Sympathetic C neurons in lumbar paravertebral ganglia of the bullfrog have previously been shown to be vasomotor in function and to express neuropeptide Y (NPY). In the present experiments, a sensitive radioimmunoassay was used to measure the NPY content of ganglia and the descending abdominal aorta and to measure the overflow of NPY evoked by depolarizing concentrations of K+. Paravertebral ganglia 9 and 10 contain 3.1 pg NPY/μg protein and the aorta contains 0.18 pg NPY/μg protein. During 20-min depolarizations in high K+ (58 mM) Ringer, the ganglia released ∼ 5% of their NPY content and the aorta released ∼ 2% of its NPY content. Pretreatment of the tissues with Ringer containing 0.18 mM Ca2+, 8 mM Mg2+, and 1 mM Co2+ blocked the NPY release elicited by high K+. These findings provide further evidence that NPY is a postganglionic co-transmitter in sympathetic C neurons of the bullfrog.

Original languageEnglish (US)
Pages (from-to)231-236
Number of pages6
JournalJournal of the Autonomic Nervous System
Volume38
Issue number3
DOIs
StatePublished - May 15 1992
Externally publishedYes

Fingerprint

Rana catesbeiana
Neuropeptide Y
Neurons
Ganglia
Aorta
Abdominal Aorta
Thoracic Aorta
Radioimmunoassay
Proteins

Keywords

  • Aorta
  • Bullfrog
  • Neuropeptide Y
  • Radioimmunoassay
  • Sympathetic neuron
  • Vasomotor neuron

ASJC Scopus subject areas

  • Physiology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Ganglionic and arterial release of neuropeptide Y by bullfrog sympathetic neurons. / Richard Thorne, M.; Smith, M (Susan); Horn, John P.

In: Journal of the Autonomic Nervous System, Vol. 38, No. 3, 15.05.1992, p. 231-236.

Research output: Contribution to journalArticle

Richard Thorne, M. ; Smith, M (Susan) ; Horn, John P. / Ganglionic and arterial release of neuropeptide Y by bullfrog sympathetic neurons. In: Journal of the Autonomic Nervous System. 1992 ; Vol. 38, No. 3. pp. 231-236.
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