Purpose: Gamma interferon (IFNγ) has been implicated in a number of inflammatory responses, including eye inflammation. With the advent of transgenic technology, we studied the involvement of IFNγ in endotoxin-induced uveitis (EIU) using IFNγ deficient mice. Methods: E.coli 055:B5 endotoxin(250 ng) was injected intravitreally in both eyes of IFNγ knockout (IFNγ KO) and BALB/cJ control mice. After 24 hours, eyes were collected for histology, and inflammation was determined by counting the number of infiltrating cells in a saggital section near the optic nerve. Results: Intravitreal injection of endotoxin in IFNγ KO mice resulted in significantly fewer infiltrating cells than in congenic controls (p=0.048). Cell counts from one IFNγ KO mouse were excluded as an outlier (>2 standard deviations from the mean). Data from a second KO mouse were excluded due to a suspected artifactual infiltrate in the ciliary body. The mean number of cells were 48.2 ± 32.9 in IFNγ KO mice (n=8) compared to 136.6 ± 88.5 in the BALB/cJ controls (n=10). Conclusion: These results are in accordance with IFNγ being a major mediator of EIU in mice.
|Original language||English (US)|
|Journal||Investigative Ophthalmology and Visual Science|
|State||Published - Feb 15 1996|
ASJC Scopus subject areas
- Sensory Systems
- Cellular and Molecular Neuroscience