Gamma interferon knockout mice show a reduction in endotoxin-induced uveitis

S. R. Planck, V. M. Lee, J. M. Park, Y. B. Han, J. T. Rosenbaum

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Purpose: Gamma interferon (IFNγ) has been implicated in a number of inflammatory responses, including eye inflammation. With the advent of transgenic technology, we studied the involvement of IFNγ in endotoxin-induced uveitis (EIU) using IFNγ deficient mice. Methods: E.coli 055:B5 endotoxin(250 ng) was injected intravitreally in both eyes of IFNγ knockout (IFNγ KO) and BALB/cJ control mice. After 24 hours, eyes were collected for histology, and inflammation was determined by counting the number of infiltrating cells in a saggital section near the optic nerve. Results: Intravitreal injection of endotoxin in IFNγ KO mice resulted in significantly fewer infiltrating cells than in congenic controls (p=0.048). Cell counts from one IFNγ KO mouse were excluded as an outlier (>2 standard deviations from the mean). Data from a second KO mouse were excluded due to a suspected artifactual infiltrate in the ciliary body. The mean number of cells were 48.2 ± 32.9 in IFNγ KO mice (n=8) compared to 136.6 ± 88.5 in the BALB/cJ controls (n=10). Conclusion: These results are in accordance with IFNγ being a major mediator of EIU in mice.

Original languageEnglish (US)
Pages (from-to)S543
JournalInvestigative Ophthalmology and Visual Science
Issue number3
StatePublished - Feb 15 1996

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience


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