Gamma-endorphin-like peptides in pituitary tissue: Evidence for their existence in vivo

Daniel M. Dorsa; Margaret B. Chapman; Denis G. Baskin

doi:10.1016/0196-9781(82)90107-3

Gamma-endorphin-like peptides in pituitary tissue: Evidence for their existence in vivo

Daniel M. Dorsa, Margaret B. Chapman, Denis G. Baskin

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Beta and gamma endorphin-like peptides were measured by radioimmunoassay in whole pituitary. Boiling of acetic acid extracts prior to tissue disruption increased the concentration of both βE- and γE-like peptides. The γE-like immunoreactivity from the neurointermediate lobe of the pituitary co-eluted with synthetic γE upon gel permeation chromatography. Immunoreactivity for βE-like and γE-like peptides in the intermediate lobe of the pituitary was also shown by immunoperoxidase staining. The results suggest that γE-like peptides are present primarily in the pars intermedia in vivo and do not arise as artifacts of acid extraction of pituitary tissue.

Original language	English (US)
Pages (from-to)	455-460
Number of pages	6
Journal	Peptides
Volume	3
Issue number	3
DOIs	https://doi.org/10.1016/0196-9781(82)90107-3
State	Published - 1982
Externally published	Yes

Keywords

Immunocytochemistry
Pituitary
Radioimmunoassay
β-Endorphin
γ-Endorphin

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

Access to Document

10.1016/0196-9781(82)90107-3

Cite this

@article{63a7d08aed05420199f5a55a7ea8ea13,

title = "Gamma-endorphin-like peptides in pituitary tissue: Evidence for their existence in vivo",

abstract = "Beta and gamma endorphin-like peptides were measured by radioimmunoassay in whole pituitary. Boiling of acetic acid extracts prior to tissue disruption increased the concentration of both βE- and γE-like peptides. The γE-like immunoreactivity from the neurointermediate lobe of the pituitary co-eluted with synthetic γE upon gel permeation chromatography. Immunoreactivity for βE-like and γE-like peptides in the intermediate lobe of the pituitary was also shown by immunoperoxidase staining. The results suggest that γE-like peptides are present primarily in the pars intermedia in vivo and do not arise as artifacts of acid extraction of pituitary tissue.",

keywords = "Immunocytochemistry, Pituitary, Radioimmunoassay, β-Endorphin, γ-Endorphin",

author = "Dorsa, {Daniel M.} and Chapman, {Margaret B.} and Baskin, {Denis G.}",

note = "Funding Information: This work was supported by the Veterans Administration, the Alcohol and Drug Abuse Institute and NIH Grant AM 17047 to the Diabetes Research Center of the University of Washington. The authors wish to thank Lucy Bottemiller, Chare Vathanaprida and Henderson Mar for skillful technical assistance.",

year = "1982",

doi = "10.1016/0196-9781(82)90107-3",

language = "English (US)",

volume = "3",

pages = "455--460",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "3",

}

TY - JOUR

T1 - Gamma-endorphin-like peptides in pituitary tissue

T2 - Evidence for their existence in vivo

AU - Dorsa, Daniel M.

AU - Chapman, Margaret B.

AU - Baskin, Denis G.

N1 - Funding Information: This work was supported by the Veterans Administration, the Alcohol and Drug Abuse Institute and NIH Grant AM 17047 to the Diabetes Research Center of the University of Washington. The authors wish to thank Lucy Bottemiller, Chare Vathanaprida and Henderson Mar for skillful technical assistance.

PY - 1982

Y1 - 1982

N2 - Beta and gamma endorphin-like peptides were measured by radioimmunoassay in whole pituitary. Boiling of acetic acid extracts prior to tissue disruption increased the concentration of both βE- and γE-like peptides. The γE-like immunoreactivity from the neurointermediate lobe of the pituitary co-eluted with synthetic γE upon gel permeation chromatography. Immunoreactivity for βE-like and γE-like peptides in the intermediate lobe of the pituitary was also shown by immunoperoxidase staining. The results suggest that γE-like peptides are present primarily in the pars intermedia in vivo and do not arise as artifacts of acid extraction of pituitary tissue.

AB - Beta and gamma endorphin-like peptides were measured by radioimmunoassay in whole pituitary. Boiling of acetic acid extracts prior to tissue disruption increased the concentration of both βE- and γE-like peptides. The γE-like immunoreactivity from the neurointermediate lobe of the pituitary co-eluted with synthetic γE upon gel permeation chromatography. Immunoreactivity for βE-like and γE-like peptides in the intermediate lobe of the pituitary was also shown by immunoperoxidase staining. The results suggest that γE-like peptides are present primarily in the pars intermedia in vivo and do not arise as artifacts of acid extraction of pituitary tissue.

KW - Immunocytochemistry

KW - Pituitary

KW - Radioimmunoassay

KW - β-Endorphin

KW - γ-Endorphin

UR - http://www.scopus.com/inward/record.url?scp=0020325105&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020325105&partnerID=8YFLogxK

U2 - 10.1016/0196-9781(82)90107-3

DO - 10.1016/0196-9781(82)90107-3

M3 - Article

C2 - 6181492

AN - SCOPUS:0020325105

SN - 0196-9781

VL - 3

SP - 455

EP - 460

JO - Peptides

JF - Peptides

IS - 3

ER -

Gamma-endorphin-like peptides in pituitary tissue: Evidence for their existence in vivo

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this