Gamma-endorphin-like peptides in pituitary tissue: Evidence for their existence in vivo

Daniel M. Dorsa, Margaret B. Chapman, Denis G. Baskin

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Beta and gamma endorphin-like peptides were measured by radioimmunoassay in whole pituitary. Boiling of acetic acid extracts prior to tissue disruption increased the concentration of both βE- and γE-like peptides. The γE-like immunoreactivity from the neurointermediate lobe of the pituitary co-eluted with synthetic γE upon gel permeation chromatography. Immunoreactivity for βE-like and γE-like peptides in the intermediate lobe of the pituitary was also shown by immunoperoxidase staining. The results suggest that γE-like peptides are present primarily in the pars intermedia in vivo and do not arise as artifacts of acid extraction of pituitary tissue.

Original languageEnglish (US)
Pages (from-to)455-460
Number of pages6
JournalPeptides
Volume3
Issue number3
DOIs
StatePublished - Jan 1 1982

Keywords

  • Immunocytochemistry
  • Pituitary
  • Radioimmunoassay
  • β-Endorphin
  • γ-Endorphin

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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