Gain of Iq25-32, 12q23-24.3, 17q12-22 facilitates tumorigenesis and progression of human squamous cell lung cancer

J. Ma, M. Gao, Y. Lu, X. Feng, J. Zhang, D. Lin, T. Xiao, Z. Hu, J. Yuan, K. Su, J. Shipley, J. Xue, Y. Gao

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

To explore the genetic changes involved in the stepwise development of lung cancer, we have determined the genetic events associated with the histological progression from normal bronchial epithelium to squamous cell carcinoma. Comparative genomic hybridization was used to identify chromosomal imbalances in 54 microdissected samples, including squamous metaplasia, dysplasia, carcinoma in situ, and invasive tumour derived from 23 patients with squamous cell carcinoma of the lung. Histopathological progression was accompanied by an increased number of chromosomal abnormalities. Gains of 1q25-32, 12q23-24.3, and 17q12-22, in particular, were detected at high frequencies in both carcinoma in situ and invasive tumours and were found more often in the cases with lymph node metastases than in those without. Our previous expression profiling of squamous cell carcinomas had identified overexpression of laminin5 γ2, a gene located at 1q25-31. Therefore, this was investigated at the protein level by inummohistochemical analysis in 336 samples of squamous cell carcinoma of the lung. Consistent with the genomic data for this region, the expression level of laminin5 γ2 was higher in the primary tumours with lymph node metastases than in tumours without metastases (p = 0.012). These data suggest that gains of genes from 1q25-32, 12q23-24.3, and 17q12-22 facilitate tumorigenesis and progression of squamous cell carcinoma of the lung, and may serve as potential predictors for this disease.

Original languageEnglish (US)
Pages (from-to)205-213
Number of pages9
JournalJournal of Pathology
Volume210
Issue number2
DOIs
StatePublished - Oct 1 2006

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Keywords

  • Comparative genomic hybridization
  • Laminin5 γ2
  • Precancerous lesion
  • Squamous cell lung cancer
  • Tissue microarrays

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Ma, J., Gao, M., Lu, Y., Feng, X., Zhang, J., Lin, D., Xiao, T., Hu, Z., Yuan, J., Su, K., Shipley, J., Xue, J., & Gao, Y. (2006). Gain of Iq25-32, 12q23-24.3, 17q12-22 facilitates tumorigenesis and progression of human squamous cell lung cancer. Journal of Pathology, 210(2), 205-213. https://doi.org/10.1002/path.2050