Gag protein from human immunodeficiency virus type 1 assembles in the absence of cyclophilin A

Daniel Streblow, Moiz Kitabwalla, C. David Pauza

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Human immunodeficiency virus type 1 (HIV-1) replication requires coordinated activities of host and viral factors. We reported previously that interactions of the host factor cyclophilin A with HIV-1 Gag polyproteins affected Gag processing and maturation of virus particles (Streblow et al., 1998. Virology 245, 197-202). We now use in vitro translation and physical analysis of Gag structures to refine our understanding of how cyclophilin A affects HIV-1 replication. Gag assembled into oligomeric structures in vitro in the presence or absence of cyclophilin A, and proteins synthesized under the two conditions were equally susceptible to cleavage by exogenous HIV-1 protease. These and previous data show that Cyclophilin A is required at a step between Gag assembly and Gag processing/virion morphogenesis. Cyclophilin A may be required for Gag conformational changes subsequent to assembly, that are required for efficient dimerization and activation of the viral protease.

Original languageEnglish (US)
Pages (from-to)228-234
Number of pages7
JournalVirology
Volume252
Issue number1
DOIs
StatePublished - Dec 5 1998
Externally publishedYes

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Cyclophilin A
gag Gene Products
HIV-1
Virus Replication
Virion
Peptide Hydrolases
Virus Activation
Virology
Dimerization
Morphogenesis
Proteins

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Gag protein from human immunodeficiency virus type 1 assembles in the absence of cyclophilin A. / Streblow, Daniel; Kitabwalla, Moiz; Pauza, C. David.

In: Virology, Vol. 252, No. 1, 05.12.1998, p. 228-234.

Research output: Contribution to journalArticle

Streblow, Daniel ; Kitabwalla, Moiz ; Pauza, C. David. / Gag protein from human immunodeficiency virus type 1 assembles in the absence of cyclophilin A. In: Virology. 1998 ; Vol. 252, No. 1. pp. 228-234.
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