Abstract
Aim: In nondividing neurons examine the role of Gadd45b in active 5-methylcytosine (5MC) and 5-hydroxymethylcytosine (5HMC) removal at a gene promoter highly implicated in mental illnesses and cognition, Bdnf. Materials & methods: Mouse primary cortical neuronal cultures with and without Gadd45b siRNA transfection were treated with N-methyl-d-aspartate (NMDA). Expression changes of genes reportedly involved in DNA demethylation, Bdnf mRNA and protein and 5MC and 5HMC at Bdnf promoters were measured. Results: Gadd45b siRNA transfection in neurons abolishes the NMDA-induced increase in Bdnf IXa mRNA and reductions in 5MC and 5HMC at the Bdnf IXa promoter. Conclusion: These results contribute to our understanding of DNA demethylation mechanisms in neurons, and its role in regulating NMDA responsive genes implicated in mental illnesses.
Original language | English (US) |
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Pages (from-to) | 567-579 |
Number of pages | 13 |
Journal | Epigenomics |
Volume | 7 |
Issue number | 4 |
DOIs | |
State | Published - Jun 1 2015 |
Keywords
- CpG
- alcoholism
- epigenetic
- histone
- schizophrenia
ASJC Scopus subject areas
- Genetics
- Cancer Research