GABAA receptor subunit profiles of tangentially migrating neurons derived from the medial ganglionic eminence

Verginia Cuzon Carlson, Hermes H. Yeh

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

During rodent corticogenesis, a sizeable subpopulation of γ-aminobutyric acid (GABA)ergic interneurons arises extracortically from the medial ganglionic eminence (MGE). These neurons progressively acquire responsiveness to GABA in the course of corticopetal tangential migration, a process regulated by ambient GABA and mediated by GABAA receptors. Here, we combined patch clamp electrophysiology and single-cell reverse transcription-polymerase chain reaction to examine GABAA receptor expression in green fluorescent MGE-derived (eGFP+) cells in telencephalic slices from gestational day 14.5 BAC-Lhx6 embryos. GABA concentration-response curves revealed lower apparent affinity and efficacy in eGFP+ cells in and around the MGE than their counterparts in the cortex. Pharmacological tests revealed subunit-selective response profiles in the MGE and cortex consistent with differential expression of GABAA receptor isoforms. Profiling of GABAA receptor subunit transcripts (α1-5, β1-3, and γ1-3, δ) uncovered increased expression of the α1-, α2-, α5-, γ2-, and γ3-subunit messenger RNAs in the cortex. We propose that the dynamic expression of certain GABAA receptor subunits contributes to assembling receptor isoforms that confer functional attributes important in regulating the migration and maturation of primordial GABAergic cortical interneurons.

Original languageEnglish (US)
Pages (from-to)1792-1802
Number of pages11
JournalCerebral Cortex
Volume21
Issue number8
DOIs
StatePublished - Aug 2011
Externally publishedYes

Fingerprint

Median Eminence
GABA-A Receptors
gamma-Aminobutyric Acid
Neurons
Interneurons
Protein Isoforms
Aminobutyrates
Telencephalon
Electrophysiology
Reverse Transcription
Rodentia
Embryonic Structures
Pharmacology
Polymerase Chain Reaction
Messenger RNA

Keywords

  • cortex
  • GABA receptor subunits
  • medial ganglionic eminence
  • single-cell expression profiling
  • tangential migration

ASJC Scopus subject areas

  • Cognitive Neuroscience
  • Cellular and Molecular Neuroscience
  • Medicine(all)

Cite this

GABAA receptor subunit profiles of tangentially migrating neurons derived from the medial ganglionic eminence. / Cuzon Carlson, Verginia; Yeh, Hermes H.

In: Cerebral Cortex, Vol. 21, No. 8, 08.2011, p. 1792-1802.

Research output: Contribution to journalArticle

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