G protein activation of a hormone-stimulated phosphatase in human tumor cells

Ming Gui Pan, Tullio Florio, Philip Stork

Research output: Contribution to journalArticle

187 Citations (Scopus)

Abstract

The growth-inhibiting peptide hormone somatostatin stimulates phosphotyrosine phosphatase activity in the human pancreatic cell line MIA PaCa-2. This hormonal activation was mediated by a pertussis toxin-sensitive guanosine 5′-triphosphate-binding protein (G protein) in the membranes of these cells. Activation of this G protein by somatostatin stimulated the dephosphorylation of exogenous epidermal growth factor receptor prepared from A-431 cells in vitro. This pathway may mediate the antineoplastic action of somatostatin in these cells and in human tumors and could represent a general mechanism of G protein coupling that is utilized by normal cells in the hormonal control of cell growth.

Original languageEnglish (US)
Pages (from-to)1215-1217
Number of pages3
JournalScience
Volume256
Issue number5060
StatePublished - May 22 1992

Fingerprint

GTP-Binding Proteins
Phosphoric Monoester Hydrolases
Hormones
Somatostatin
Neoplasms
Protein Tyrosine Phosphatases
Somatostatin-Secreting Cells
Peptide Hormones
Pertussis Toxin
Growth
Guanosine Triphosphate
Epidermal Growth Factor Receptor
Human Activities
Antineoplastic Agents
Carrier Proteins
Cell Membrane
Cell Line

ASJC Scopus subject areas

  • General

Cite this

G protein activation of a hormone-stimulated phosphatase in human tumor cells. / Pan, Ming Gui; Florio, Tullio; Stork, Philip.

In: Science, Vol. 256, No. 5060, 22.05.1992, p. 1215-1217.

Research output: Contribution to journalArticle

Pan, MG, Florio, T & Stork, P 1992, 'G protein activation of a hormone-stimulated phosphatase in human tumor cells', Science, vol. 256, no. 5060, pp. 1215-1217.
Pan, Ming Gui ; Florio, Tullio ; Stork, Philip. / G protein activation of a hormone-stimulated phosphatase in human tumor cells. In: Science. 1992 ; Vol. 256, No. 5060. pp. 1215-1217.
@article{fa60b5c4e5eb4ccab2e2d197d6d997f4,
title = "G protein activation of a hormone-stimulated phosphatase in human tumor cells",
abstract = "The growth-inhibiting peptide hormone somatostatin stimulates phosphotyrosine phosphatase activity in the human pancreatic cell line MIA PaCa-2. This hormonal activation was mediated by a pertussis toxin-sensitive guanosine 5′-triphosphate-binding protein (G protein) in the membranes of these cells. Activation of this G protein by somatostatin stimulated the dephosphorylation of exogenous epidermal growth factor receptor prepared from A-431 cells in vitro. This pathway may mediate the antineoplastic action of somatostatin in these cells and in human tumors and could represent a general mechanism of G protein coupling that is utilized by normal cells in the hormonal control of cell growth.",
author = "Pan, {Ming Gui} and Tullio Florio and Philip Stork",
year = "1992",
month = "5",
day = "22",
language = "English (US)",
volume = "256",
pages = "1215--1217",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "5060",

}

TY - JOUR

T1 - G protein activation of a hormone-stimulated phosphatase in human tumor cells

AU - Pan, Ming Gui

AU - Florio, Tullio

AU - Stork, Philip

PY - 1992/5/22

Y1 - 1992/5/22

N2 - The growth-inhibiting peptide hormone somatostatin stimulates phosphotyrosine phosphatase activity in the human pancreatic cell line MIA PaCa-2. This hormonal activation was mediated by a pertussis toxin-sensitive guanosine 5′-triphosphate-binding protein (G protein) in the membranes of these cells. Activation of this G protein by somatostatin stimulated the dephosphorylation of exogenous epidermal growth factor receptor prepared from A-431 cells in vitro. This pathway may mediate the antineoplastic action of somatostatin in these cells and in human tumors and could represent a general mechanism of G protein coupling that is utilized by normal cells in the hormonal control of cell growth.

AB - The growth-inhibiting peptide hormone somatostatin stimulates phosphotyrosine phosphatase activity in the human pancreatic cell line MIA PaCa-2. This hormonal activation was mediated by a pertussis toxin-sensitive guanosine 5′-triphosphate-binding protein (G protein) in the membranes of these cells. Activation of this G protein by somatostatin stimulated the dephosphorylation of exogenous epidermal growth factor receptor prepared from A-431 cells in vitro. This pathway may mediate the antineoplastic action of somatostatin in these cells and in human tumors and could represent a general mechanism of G protein coupling that is utilized by normal cells in the hormonal control of cell growth.

UR - http://www.scopus.com/inward/record.url?scp=0026682699&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026682699&partnerID=8YFLogxK

M3 - Article

VL - 256

SP - 1215

EP - 1217

JO - Science

JF - Science

SN - 0036-8075

IS - 5060

ER -