Fusion of protoplasts of streptomyces lavendulae

Michiko M. Nakano; Hiroshi Ishihara; Hiroshi Ogawara

doi:10.7164/antibiotics.35.359

Fusion of protoplasts of streptomyces lavendulae

Michiko M. Nakano, Hiroshi Ishihara, Hiroshi Ogawara

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Protoplasts of two different auxotrophic mutants of Streptomyces lavendulae were fused with polyethylene glycol (PEG) 1000, and allowed to regenerate on selective media. Prototrophic colonies overwhelmed other types of recombinants on any selective media. These prototrophic strains were stable after successive isolation. These results suggest that stable diploid cells were formed by cell fusion, which differed from the case of S. coelicolor.

Original language	English (US)
Pages (from-to)	359-363
Number of pages	5
Journal	the journal of antibiotics
Volume	35
Issue number	3
DOIs	https://doi.org/10.7164/antibiotics.35.359
State	Published - 1982
Externally published	Yes

ASJC Scopus subject areas

Pharmacology
Drug Discovery

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title = "Fusion of protoplasts of streptomyces lavendulae",

abstract = "Protoplasts of two different auxotrophic mutants of Streptomyces lavendulae were fused with polyethylene glycol (PEG) 1000, and allowed to regenerate on selective media. Prototrophic colonies overwhelmed other types of recombinants on any selective media. These prototrophic strains were stable after successive isolation. These results suggest that stable diploid cells were formed by cell fusion, which differed from the case of S. coelicolor.",

author = "Nakano, {Michiko M.} and Hiroshi Ishihara and Hiroshi Ogawara",

year = "1982",

doi = "10.7164/antibiotics.35.359",

language = "English (US)",

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pages = "359--363",

journal = "the journal of antibiotics",

issn = "0021-8820",

publisher = "Japan Antibiotics Research Association",

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T1 - Fusion of protoplasts of streptomyces lavendulae

AU - Nakano, Michiko M.

AU - Ishihara, Hiroshi

AU - Ogawara, Hiroshi

PY - 1982

Y1 - 1982

N2 - Protoplasts of two different auxotrophic mutants of Streptomyces lavendulae were fused with polyethylene glycol (PEG) 1000, and allowed to regenerate on selective media. Prototrophic colonies overwhelmed other types of recombinants on any selective media. These prototrophic strains were stable after successive isolation. These results suggest that stable diploid cells were formed by cell fusion, which differed from the case of S. coelicolor.

AB - Protoplasts of two different auxotrophic mutants of Streptomyces lavendulae were fused with polyethylene glycol (PEG) 1000, and allowed to regenerate on selective media. Prototrophic colonies overwhelmed other types of recombinants on any selective media. These prototrophic strains were stable after successive isolation. These results suggest that stable diploid cells were formed by cell fusion, which differed from the case of S. coelicolor.

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JO - the journal of antibiotics

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ER -

Fusion of protoplasts of streptomyces lavendulae

Abstract

ASJC Scopus subject areas

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