Further refinement of the Escherichia coli brain abscess model in rat

Jules M. Nazzaro, Michael A. Pagel, Edward A. Neuwelt

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

The rat brain abscess model provides a substrate for the modeling of delivery of therapeutic agents to intracerebral mass lesions. We now report refinement of the Escherichia coli brain abscess model in rat. A K1 surface antigen-negative E. coli isolated from human blood culture was stereotaxically inoculated into deep brain sites. Histopathologic analyses and quantitative cultures demonstrated the consistent production of lesions. No animal in this consecutive series developed meningitis, ventriculitis or sepsis. By contrast, prior experience with E. coli abscess production resulted in 25% failure rate of abscess production or death from sepsis. This improvement in the model may be attributable to specific characteristics of the bacteria used, modification of the inoculation method or the intracerebral placement technique. The present work suggests a reliable and consistent brain abscess model, which may be further used to study brain suppuration.

Original languageEnglish (US)
Pages (from-to)85-90
Number of pages6
JournalJournal of Neuroscience Methods
Volume44
Issue number2-3
DOIs
StatePublished - Sep 1992

Keywords

  • (Rat)
  • Animal brain abscess model
  • Brain abscess
  • Brain inoculation technique
  • Escherichia coli

ASJC Scopus subject areas

  • Neuroscience(all)

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