Further characterization of the keratinocyte somatomedin-C/insuIin-like growth factor I (SM-C/IGF-I) receptor and the biological responsiveness of cultured keratinocytes to SM-C/IGF-I

Brian J. Nickoloff; Pratima Misra; Vera B. Morhenn; Raymond L. Hintz; Ron G. Rosenfeld

doi:10.1159/000248577

Further characterization of the keratinocyte somatomedin-C/insuIin-like growth factor I (SM-C/IGF-I) receptor and the biological responsiveness of cultured keratinocytes to SM-C/IGF-I

Brian J. Nickoloff, Pratima Misra, Vera B. Morhenn, Raymond L. Hintz, Ron G. Rosenfeld

Pediatrics

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Somatomedin-C/insulin-likc growth factor I (SM-C/IGF-I) binds to both cultured keratinocytes trypsinized to single-cell suspensions and fresh epidermal sheets derived from 1 MNaBr-treated 6-mm punch biopsies. A gradual increase of 1-4% in specific binding of SM-C/IGF-1 to fresh epidermal sheets was observed during a 5-hour incubation period at 15 °C. The human keratinocyte SM-C/IGF-I receptor was isolated by polyacrylamide gel electrophoresis after cross-linking with iodinated SM-C/IGF-I and migrated at 135,000 daltons under reducing conditions. Both a partially purified preparation of SM-C/IGF-I, as well as recombinant DNA-derived thr-59-SM-C/IGF-I (25-100 ng/ml), stimulated keratinocyte replication in a serum-free system, with an approximately equivalent effect as insulin (10 μg/ml). Since fibroblasts produce SM-C/IGF-I, our data suggest that some dermal-epidermal interactions in diseases such as psoriasis may be mediated, in part, by SM-C/IGF-I.

Original language	English (US)
Pages (from-to)	265-273
Number of pages	9
Journal	Dermatology
Volume	177
Issue number	5
DOIs	https://doi.org/10.1159/000248577
State	Published - 1988

Keywords

Insulin-like growth factor I
Psoriasis
Somatomedin-C

ASJC Scopus subject areas

Dermatology

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Further characterization of the keratinocyte somatomedin-C/insuIin-like growth factor I (SM-C/IGF-I) receptor and the biological responsiveness of cultured keratinocytes to SM-C/IGF-I. / Nickoloff, Brian J.; Misra, Pratima; Morhenn, Vera B.; Hintz, Raymond L.; Rosenfeld, Ron G.

In: Dermatology, Vol. 177, No. 5, 1988, p. 265-273.

Research output: Contribution to journal › Article › peer-review

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abstract = "Somatomedin-C/insulin-likc growth factor I (SM-C/IGF-I) binds to both cultured keratinocytes trypsinized to single-cell suspensions and fresh epidermal sheets derived from 1 MNaBr-treated 6-mm punch biopsies. A gradual increase of 1-4% in specific binding of SM-C/IGF-1 to fresh epidermal sheets was observed during a 5-hour incubation period at 15 °C. The human keratinocyte SM-C/IGF-I receptor was isolated by polyacrylamide gel electrophoresis after cross-linking with iodinated SM-C/IGF-I and migrated at 135,000 daltons under reducing conditions. Both a partially purified preparation of SM-C/IGF-I, as well as recombinant DNA-derived thr-59-SM-C/IGF-I (25-100 ng/ml), stimulated keratinocyte replication in a serum-free system, with an approximately equivalent effect as insulin (10 μg/ml). Since fibroblasts produce SM-C/IGF-I, our data suggest that some dermal-epidermal interactions in diseases such as psoriasis may be mediated, in part, by SM-C/IGF-I.",

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AB - Somatomedin-C/insulin-likc growth factor I (SM-C/IGF-I) binds to both cultured keratinocytes trypsinized to single-cell suspensions and fresh epidermal sheets derived from 1 MNaBr-treated 6-mm punch biopsies. A gradual increase of 1-4% in specific binding of SM-C/IGF-1 to fresh epidermal sheets was observed during a 5-hour incubation period at 15 °C. The human keratinocyte SM-C/IGF-I receptor was isolated by polyacrylamide gel electrophoresis after cross-linking with iodinated SM-C/IGF-I and migrated at 135,000 daltons under reducing conditions. Both a partially purified preparation of SM-C/IGF-I, as well as recombinant DNA-derived thr-59-SM-C/IGF-I (25-100 ng/ml), stimulated keratinocyte replication in a serum-free system, with an approximately equivalent effect as insulin (10 μg/ml). Since fibroblasts produce SM-C/IGF-I, our data suggest that some dermal-epidermal interactions in diseases such as psoriasis may be mediated, in part, by SM-C/IGF-I.

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Further characterization of the keratinocyte somatomedin-C/insuIin-like growth factor I (SM-C/IGF-I) receptor and the biological responsiveness of cultured keratinocytes to SM-C/IGF-I

Abstract

Keywords

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