Functionally specific optogenetic modulation in primate visual cortex

Mykyta M. Chernov, Robert M. Friedman, Gang Chen, Gene R. Stoner, Anna Roe

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In primates, visual perception is mediated by brain circuits composed of submillimeter nodes linked together in specific networks that process different types of information, such as eye specificity and contour orientation. We hypothesized that optogenetic stimulation targeted to cortical nodes could selectively activate such cortical networks. We used viral transfection methods to confer light sensitivity to neurons in monkey primary visual cortex. Using intrinsic signal optical imaging and single-unit electrophysiology to assess effects of targeted optogenetic stimulation, we found that (i) optogenetic stimulation of single ocular dominance columns (eye-specific nodes) revealed preferential activation of nearby same-eye columns but not opposite-eye columns, and (ii) optogenetic stimulation of single orientation domains increased visual response of matching orientation domains and relatively suppressed nonmatching orientation selectivity. These findings demonstrate that optical stimulation of single nodes leads to modulation of functionally specific cortical networks related to underlying neural architecture.

Original languageEnglish (US)
Pages (from-to)10505-10510
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume115
Issue number41
DOIs
StatePublished - Oct 9 2018

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Optogenetics
Visual Cortex
Primates
ocular Dominance
Photophobia
Visual Perception
Electrophysiology
Optical Imaging
Haplorhini
Transfection
Neurons
Brain

ASJC Scopus subject areas

  • General

Cite this

Functionally specific optogenetic modulation in primate visual cortex. / Chernov, Mykyta M.; Friedman, Robert M.; Chen, Gang; Stoner, Gene R.; Roe, Anna.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 115, No. 41, 09.10.2018, p. 10505-10510.

Research output: Contribution to journalArticle

Chernov, Mykyta M. ; Friedman, Robert M. ; Chen, Gang ; Stoner, Gene R. ; Roe, Anna. / Functionally specific optogenetic modulation in primate visual cortex. In: Proceedings of the National Academy of Sciences of the United States of America. 2018 ; Vol. 115, No. 41. pp. 10505-10510.
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