Functional subsets within clonally expanded CD8+ memory T cells in elderly humans

Winston Chamberlain, Michael T. Falta, Brian L. Kotzin

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

With advancing age, healthy humans frequently demonstrate large clonal expansions of CD8+ T cells in the peripheral blood, which persist for long periods of time and appear to be maintained as a population of memory cells. We studied nine large T cell clones in five elderly individuals. We noted that in most cases the expanded clones were dominated by cells that did not express CD28, a pivotal molecule in T cell activation, and these clones proliferated poorly in culture. However, nearly all of the clonal expansions had CD28+ fractions and some of these cells appeared to lose CD28 gene expression with stimulation in culture. CD28+ cells demonstrated greater proliferation in both bulk and limiting dilution cultures compared to CD28- cells bearing the same TCR, whereas CD28- cells showed increased perforin expression. Together, these data suggest that loss of CD28 expression marks functional differentiation to cytotoxic memory cells within these clonal expansions and likely within CD8+ memory populations in general. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)160-172
Number of pages13
JournalClinical Immunology
Volume94
Issue number3
DOIs
StatePublished - Mar 2000
Externally publishedYes

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T-Lymphocytes
Clone Cells
Perforin
Population
Gene Expression

Keywords

  • Memory cells, CD8 T lymphocytes, CD28 expression
  • T cell clonal expansions
  • T lymphocytes

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pathology and Forensic Medicine

Cite this

Functional subsets within clonally expanded CD8+ memory T cells in elderly humans. / Chamberlain, Winston; Falta, Michael T.; Kotzin, Brian L.

In: Clinical Immunology, Vol. 94, No. 3, 03.2000, p. 160-172.

Research output: Contribution to journalArticle

Chamberlain, Winston ; Falta, Michael T. ; Kotzin, Brian L. / Functional subsets within clonally expanded CD8+ memory T cells in elderly humans. In: Clinical Immunology. 2000 ; Vol. 94, No. 3. pp. 160-172.
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