Functional selectivity of recombinant mammalian SWI/SNF subunits

Shilpa Kadam, Glenn S. McAlpine, Michael L. Phelan, Robert E. Kingston, Katherine A. Jones, Beverly Emerson

Research output: Contribution to journalArticle

173 Citations (Scopus)

Abstract

The SWI/SNF family of chromatin-remodeling complexes plays a key role in facilitating the binding of specific transcription factors to nucleosomal DNA in diverse organisms from yeast to man. Yet the process by which SWI/SNF and other chromatin-remodeling complexes activate specific subsets of genes is poorly understood. We show that mammalian SWI/SNF regulates transcription from chromatin-assembled genes in a factor-specific manner in vitro. The DNA-binding domains (DBDs) of several zinc finger proteins, including EKLF, interact directly with SWI/SNF to generate DNase I hypersensitivity within the chromatin-assembled β-globin promoter. Interestingly, we find that two SWI/SNF subunits (BRG1 and BAF155) are necessary and sufficient for targeted chromatin remodeling and transcriptional activation by EKLF in vitro. Remodeling is achieved with only the BRG1-BAF155 minimal complex and the EKLF zinc finger DBD, whereas transcription requires, in addition, an activation domain. In contrast, the BRG1-BAF155 complex does not interact or function with two unrelated transcription factors, TFE3 and NF-κB. We conclude that specific domains of certain transcription factors differentially target SWI/SNF complexes to chromatin in a gene-selective manner and that individual SWI/SNF subunits play unique roles in transcription factor-directed nucleosome remodeling.

Original languageEnglish (US)
Pages (from-to)2441-2451
Number of pages11
JournalGenes and Development
Volume14
Issue number19
DOIs
StatePublished - Oct 1 2000
Externally publishedYes

Fingerprint

Chromatin Assembly and Disassembly
Transcription Factors
Chromatin
Zinc Fingers
DNA
Genes
Globins
Nucleosomes
Deoxyribonuclease I
Transcriptional Activation
Hypersensitivity
Yeasts
erythroid Kruppel-like factor
Proteins
In Vitro Techniques

Keywords

  • β-globin
  • Chromatin
  • EKLF
  • SWI/SNF
  • Transcription
  • Zinc fingers

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Kadam, S., McAlpine, G. S., Phelan, M. L., Kingston, R. E., Jones, K. A., & Emerson, B. (2000). Functional selectivity of recombinant mammalian SWI/SNF subunits. Genes and Development, 14(19), 2441-2451. https://doi.org/10.1101/gad.828000

Functional selectivity of recombinant mammalian SWI/SNF subunits. / Kadam, Shilpa; McAlpine, Glenn S.; Phelan, Michael L.; Kingston, Robert E.; Jones, Katherine A.; Emerson, Beverly.

In: Genes and Development, Vol. 14, No. 19, 01.10.2000, p. 2441-2451.

Research output: Contribution to journalArticle

Kadam, S, McAlpine, GS, Phelan, ML, Kingston, RE, Jones, KA & Emerson, B 2000, 'Functional selectivity of recombinant mammalian SWI/SNF subunits', Genes and Development, vol. 14, no. 19, pp. 2441-2451. https://doi.org/10.1101/gad.828000
Kadam S, McAlpine GS, Phelan ML, Kingston RE, Jones KA, Emerson B. Functional selectivity of recombinant mammalian SWI/SNF subunits. Genes and Development. 2000 Oct 1;14(19):2441-2451. https://doi.org/10.1101/gad.828000
Kadam, Shilpa ; McAlpine, Glenn S. ; Phelan, Michael L. ; Kingston, Robert E. ; Jones, Katherine A. ; Emerson, Beverly. / Functional selectivity of recombinant mammalian SWI/SNF subunits. In: Genes and Development. 2000 ; Vol. 14, No. 19. pp. 2441-2451.
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