Functional regions in the 5 external transcribed spacer of yeast pre-rRNA

Jing Chen, Liman Zhang, Keqiong Ye

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Ribosomal subunits are assembled on a precursor rRNA that includes four spacers in addition to mature rRNA sequences. The 5′′ external transcribed spacer (5′′ ETS) is the most prominent one that recruits U3 snoRNA and a plethora of proteins during the early assembly of 90S small subunit preribosomes. Here, we have conducted a comprehensive mutational analysis of 5′′ ETS by monitoring the processing and assembly of a plasmid-expressed pre-18S RNA. Remarkably, nearly half of the 5′′ ETS sequences, when depleted individually, are dispensable for 18S rRNA processing. The dispensable elements largely bind at the surface of the 90S structure. Defective assembly of 5′′ ETS completely blocks the last stage of 90S formation yet has little effect on the early assembly of 5′′ and central domains of 18S rRNA. Our study reveals the functional regions of 5′′ ETS and provides new insight into the assembly hierarchy of 90S preribosomes.

Original languageEnglish (US)
Pages (from-to)866-877
Number of pages12
JournalRNA
Volume26
Issue number7
DOIs
StatePublished - Jul 2020
Externally publishedYes

Keywords

  • 90S preribosome
  • RRNA processing
  • Ribosome assembly

ASJC Scopus subject areas

  • Molecular Biology

Fingerprint

Dive into the research topics of 'Functional regions in the 5<sup>′</sup> external transcribed spacer of yeast pre-rRNA'. Together they form a unique fingerprint.

Cite this