Functional recovery of the developing rat ovary after transplantation: Contribution of the extrinsic innervation

H. E. Lara, W. L. Dees, J. K. Hiney, Gregory Dissen, C. Rivier, Sergio Ojeda

    Research output: Contribution to journalArticle

    43 Citations (Scopus)

    Abstract

    These experiments were undertaken to define the neuroendocrine mechanisms underlying the recovery of ovarian function after transplantation to an ectopic site. Both ovaries from 23-day-old rats were transplanted to the region of the neck, next to the jugular vein. Serum gonadotropin and plasma immunoreactive inhibin-α levels were determined at several intervals thereafter. Serum estradiol (E2) was measured during the first week posttransplantation. Reinnervation of the ovary by sympathetic and sensory nerves was monitored by immunohistochemistry. Sympathetic nerves were identified as adrenergic by the presence of tyrosine hydroxylase (TH), the rate-limiting enzyme in catecholamine biosynthesis, and as peptidergic, by their neuropeptide-Y (NPY) or vasoactive intestinal peptide (VIP) immunoreactivity. Sensory nerves were identified by the presence of substance P (SP) and calcitonin-gene related peptide (CGRP) immunoreactivity. Serum LH and FSH increased, and plasma inhibin levels decreased, within 48 h after transplantation. Serum LH reached maximum levels on day 4, decreasing rapidly thereafter to basal values by day 6. These changes were functionally correlated with the posttransplantation fluctuations in serum E2, which decreased at 48 h, rebounded by day 4, and returned to basal values on day 7. Removal of the transplanted ovaries on day 3 resulted in the disappearance of serum E2 levels on day 4, thus confirming the ovarian graft as the source of E2. In contrast to LH, serum FSH remained significantly elevated for at least 3 weeks after transplantation, then decreased to basal levels after day 21, coinciding with the rise in inhibin secretion. Although a substantial loss of follicles was noted 48 h after transplantation, quantitative examination of the changes on day 4 revealed that approximately 40% of antral follicles were not necrotic. Ovulation and formation of corpora lutea were noted 21 days after transplantation. Reinnervation of the transplanted ovary by TH-, VIP-, NPY-, SP-, and CGRP-containing fibers was first detected 7 days after transplantation. Although VIP reinnervation was sparse and only transiently detected (days 7-21), the density of sympathetic (TH, NPY) and sensory (SP, CGRP) fibers increased 2- to 3-fold between days 7-28, remaining unchanged thereafter. Since apparent completion of this reinnervation coincided with reestablishment of normal levels of both LH and FSH, an additional experiment was performed to determine if the two events were causally related. Development of the sympathetic innervation of the ovary was prevented by administration of antibodies to nerve growth factor during neonatal life, and the ovaries were autotransplanted as before. Examination of the ovaries 35 days after transplantation revealed that sympathetic reinnervation had failed to occur and that sensory reinnervation was reduced. Although the capacity to ovulate was maintained, as judged by the presence of corpora lutea, basal serum LH and FSH levels did not return to pretransplantation levels, indicating failure of the ovary to fully reestablish negative feedback control on gonadotropin secretion. The results suggest that reinnervation contributes to the process by which the ovary recovers its functional competence after transplantation to an ectopic site.

    Original languageEnglish (US)
    Pages (from-to)1849-1860
    Number of pages12
    JournalEndocrinology
    Volume129
    Issue number4
    StatePublished - Oct 1991

    Fingerprint

    Ovary
    Transplantation
    Serum
    Inhibins
    Calcitonin Gene-Related Peptide
    Neuropeptide Y
    Vasoactive Intestinal Peptide
    Tyrosine 3-Monooxygenase
    Substance P
    Corpus Luteum
    Gonadotropins
    Jugular Veins
    Recovery of Function
    Nerve Growth Factor
    Ovulation
    Adrenergic Agents
    Mental Competency
    Catecholamines
    Estradiol
    Neck

    ASJC Scopus subject areas

    • Endocrinology
    • Endocrinology, Diabetes and Metabolism

    Cite this

    Lara, H. E., Dees, W. L., Hiney, J. K., Dissen, G., Rivier, C., & Ojeda, S. (1991). Functional recovery of the developing rat ovary after transplantation: Contribution of the extrinsic innervation. Endocrinology, 129(4), 1849-1860.

    Functional recovery of the developing rat ovary after transplantation : Contribution of the extrinsic innervation. / Lara, H. E.; Dees, W. L.; Hiney, J. K.; Dissen, Gregory; Rivier, C.; Ojeda, Sergio.

    In: Endocrinology, Vol. 129, No. 4, 10.1991, p. 1849-1860.

    Research output: Contribution to journalArticle

    Lara, HE, Dees, WL, Hiney, JK, Dissen, G, Rivier, C & Ojeda, S 1991, 'Functional recovery of the developing rat ovary after transplantation: Contribution of the extrinsic innervation', Endocrinology, vol. 129, no. 4, pp. 1849-1860.
    Lara, H. E. ; Dees, W. L. ; Hiney, J. K. ; Dissen, Gregory ; Rivier, C. ; Ojeda, Sergio. / Functional recovery of the developing rat ovary after transplantation : Contribution of the extrinsic innervation. In: Endocrinology. 1991 ; Vol. 129, No. 4. pp. 1849-1860.
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    abstract = "These experiments were undertaken to define the neuroendocrine mechanisms underlying the recovery of ovarian function after transplantation to an ectopic site. Both ovaries from 23-day-old rats were transplanted to the region of the neck, next to the jugular vein. Serum gonadotropin and plasma immunoreactive inhibin-α levels were determined at several intervals thereafter. Serum estradiol (E2) was measured during the first week posttransplantation. Reinnervation of the ovary by sympathetic and sensory nerves was monitored by immunohistochemistry. Sympathetic nerves were identified as adrenergic by the presence of tyrosine hydroxylase (TH), the rate-limiting enzyme in catecholamine biosynthesis, and as peptidergic, by their neuropeptide-Y (NPY) or vasoactive intestinal peptide (VIP) immunoreactivity. Sensory nerves were identified by the presence of substance P (SP) and calcitonin-gene related peptide (CGRP) immunoreactivity. Serum LH and FSH increased, and plasma inhibin levels decreased, within 48 h after transplantation. Serum LH reached maximum levels on day 4, decreasing rapidly thereafter to basal values by day 6. These changes were functionally correlated with the posttransplantation fluctuations in serum E2, which decreased at 48 h, rebounded by day 4, and returned to basal values on day 7. Removal of the transplanted ovaries on day 3 resulted in the disappearance of serum E2 levels on day 4, thus confirming the ovarian graft as the source of E2. In contrast to LH, serum FSH remained significantly elevated for at least 3 weeks after transplantation, then decreased to basal levels after day 21, coinciding with the rise in inhibin secretion. Although a substantial loss of follicles was noted 48 h after transplantation, quantitative examination of the changes on day 4 revealed that approximately 40{\%} of antral follicles were not necrotic. Ovulation and formation of corpora lutea were noted 21 days after transplantation. Reinnervation of the transplanted ovary by TH-, VIP-, NPY-, SP-, and CGRP-containing fibers was first detected 7 days after transplantation. Although VIP reinnervation was sparse and only transiently detected (days 7-21), the density of sympathetic (TH, NPY) and sensory (SP, CGRP) fibers increased 2- to 3-fold between days 7-28, remaining unchanged thereafter. Since apparent completion of this reinnervation coincided with reestablishment of normal levels of both LH and FSH, an additional experiment was performed to determine if the two events were causally related. Development of the sympathetic innervation of the ovary was prevented by administration of antibodies to nerve growth factor during neonatal life, and the ovaries were autotransplanted as before. Examination of the ovaries 35 days after transplantation revealed that sympathetic reinnervation had failed to occur and that sensory reinnervation was reduced. Although the capacity to ovulate was maintained, as judged by the presence of corpora lutea, basal serum LH and FSH levels did not return to pretransplantation levels, indicating failure of the ovary to fully reestablish negative feedback control on gonadotropin secretion. The results suggest that reinnervation contributes to the process by which the ovary recovers its functional competence after transplantation to an ectopic site.",
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    AU - Lara, H. E.

    AU - Dees, W. L.

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    N2 - These experiments were undertaken to define the neuroendocrine mechanisms underlying the recovery of ovarian function after transplantation to an ectopic site. Both ovaries from 23-day-old rats were transplanted to the region of the neck, next to the jugular vein. Serum gonadotropin and plasma immunoreactive inhibin-α levels were determined at several intervals thereafter. Serum estradiol (E2) was measured during the first week posttransplantation. Reinnervation of the ovary by sympathetic and sensory nerves was monitored by immunohistochemistry. Sympathetic nerves were identified as adrenergic by the presence of tyrosine hydroxylase (TH), the rate-limiting enzyme in catecholamine biosynthesis, and as peptidergic, by their neuropeptide-Y (NPY) or vasoactive intestinal peptide (VIP) immunoreactivity. Sensory nerves were identified by the presence of substance P (SP) and calcitonin-gene related peptide (CGRP) immunoreactivity. Serum LH and FSH increased, and plasma inhibin levels decreased, within 48 h after transplantation. Serum LH reached maximum levels on day 4, decreasing rapidly thereafter to basal values by day 6. These changes were functionally correlated with the posttransplantation fluctuations in serum E2, which decreased at 48 h, rebounded by day 4, and returned to basal values on day 7. Removal of the transplanted ovaries on day 3 resulted in the disappearance of serum E2 levels on day 4, thus confirming the ovarian graft as the source of E2. In contrast to LH, serum FSH remained significantly elevated for at least 3 weeks after transplantation, then decreased to basal levels after day 21, coinciding with the rise in inhibin secretion. Although a substantial loss of follicles was noted 48 h after transplantation, quantitative examination of the changes on day 4 revealed that approximately 40% of antral follicles were not necrotic. Ovulation and formation of corpora lutea were noted 21 days after transplantation. Reinnervation of the transplanted ovary by TH-, VIP-, NPY-, SP-, and CGRP-containing fibers was first detected 7 days after transplantation. Although VIP reinnervation was sparse and only transiently detected (days 7-21), the density of sympathetic (TH, NPY) and sensory (SP, CGRP) fibers increased 2- to 3-fold between days 7-28, remaining unchanged thereafter. Since apparent completion of this reinnervation coincided with reestablishment of normal levels of both LH and FSH, an additional experiment was performed to determine if the two events were causally related. Development of the sympathetic innervation of the ovary was prevented by administration of antibodies to nerve growth factor during neonatal life, and the ovaries were autotransplanted as before. Examination of the ovaries 35 days after transplantation revealed that sympathetic reinnervation had failed to occur and that sensory reinnervation was reduced. Although the capacity to ovulate was maintained, as judged by the presence of corpora lutea, basal serum LH and FSH levels did not return to pretransplantation levels, indicating failure of the ovary to fully reestablish negative feedback control on gonadotropin secretion. The results suggest that reinnervation contributes to the process by which the ovary recovers its functional competence after transplantation to an ectopic site.

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