Functional neurolobectomy induced by controlled compression of the pituitary stalk

Janos Dohanics, Gloria E. Hoffman, M (Susan) Smith, Joseph G. Verbalis

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Degeneration of magnocellular nerve terminals in the neurohypophysis was induced by compressing the pituitary stalk of anesthetized rats for 30 s using a triangle-shaped wire. Immediately after stalk compression (SC), rats exhibited markedly increased water intake characteristic of diabetes insipidus, followed by a triphasic pattern of fluid intake. In SC rats, arginine vasopressin (AVP) and oxitocin (OT) contents of the neurointermediate lobe (NIL) of the pituitary gland were significantly reduced to ∼2.5% and ∼10% of sham-operated controls, respectivel In contrast, OT, but not AVP, content of the stalk-median eminence (SME) of SC rats was significantly increased. Histological examination of the pituitaries showed substantial degeneration of the neural lobe with very scarce AVP-neurophysin and OT-neurophysin immunoreactivity, while both the anterior and the intermediate lobes appeared to be intact. Plasma AVP and OT responses to infusion of hypertonic NaCl were significantly blunted in SC rats compared to sham-operated controls. However, two days after surgery the secretory patterns of LH in SC rats were similar to those in the controls. These results indicate that controlled compression of the pituitary stalk results in selective degeneration of the neural lobe without causing permanent ischemic damage to the anterior pituitary, and produces marked sustained functional deficits in pituitary AVP and OT secretion. Consequently, SC provides an alternatiove means to achieve selective neurolobectomy in rats.

Original languageEnglish (US)
Pages (from-to)215-222
Number of pages8
JournalBrain Research
Volume575
Issue number2
DOIs
StatePublished - Mar 20 1992
Externally publishedYes

Fingerprint

Pituitary Gland
Arginine Vasopressin
Posterior Pituitary Gland
Neurophysins
Nerve Degeneration
Median Eminence
Diabetes Insipidus
Ambulatory Surgical Procedures
Drinking

Keywords

  • Adenohypophysis
  • Neurohypophysis
  • Oxytocin
  • Pituitary stalk
  • Vasopressin

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Functional neurolobectomy induced by controlled compression of the pituitary stalk. / Dohanics, Janos; Hoffman, Gloria E.; Smith, M (Susan); Verbalis, Joseph G.

In: Brain Research, Vol. 575, No. 2, 20.03.1992, p. 215-222.

Research output: Contribution to journalArticle

Dohanics, Janos ; Hoffman, Gloria E. ; Smith, M (Susan) ; Verbalis, Joseph G. / Functional neurolobectomy induced by controlled compression of the pituitary stalk. In: Brain Research. 1992 ; Vol. 575, No. 2. pp. 215-222.
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