Abstract
Degeneration of magnocellular nerve terminals in the neurohypophysis was induced by compressing the pituitary stalk of anesthetized rats for 30 s using a triangle-shaped wire. Immediately after stalk compression (SC), rats exhibited markedly increased water intake characteristic of diabetes insipidus, followed by a triphasic pattern of fluid intake. In SC rats, arginine vasopressin (AVP) and oxitocin (OT) contents of the neurointermediate lobe (NIL) of the pituitary gland were significantly reduced to ∼2.5% and ∼10% of sham-operated controls, respectivel In contrast, OT, but not AVP, content of the stalk-median eminence (SME) of SC rats was significantly increased. Histological examination of the pituitaries showed substantial degeneration of the neural lobe with very scarce AVP-neurophysin and OT-neurophysin immunoreactivity, while both the anterior and the intermediate lobes appeared to be intact. Plasma AVP and OT responses to infusion of hypertonic NaCl were significantly blunted in SC rats compared to sham-operated controls. However, two days after surgery the secretory patterns of LH in SC rats were similar to those in the controls. These results indicate that controlled compression of the pituitary stalk results in selective degeneration of the neural lobe without causing permanent ischemic damage to the anterior pituitary, and produces marked sustained functional deficits in pituitary AVP and OT secretion. Consequently, SC provides an alternatiove means to achieve selective neurolobectomy in rats.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 215-222 |
| Number of pages | 8 |
| Journal | Brain Research |
| Volume | 575 |
| Issue number | 2 |
| DOIs | |
| State | Published - Mar 20 1992 |
| Externally published | Yes |
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Keywords
- Adenohypophysis
- Neurohypophysis
- Oxytocin
- Pituitary stalk
- Vasopressin
ASJC Scopus subject areas
- Developmental Biology
- Molecular Biology
- Clinical Neurology
- Neuroscience(all)
Cite this
Functional neurolobectomy induced by controlled compression of the pituitary stalk. / Dohanics, Janos; Hoffman, Gloria E.; Smith, M (Susan); Verbalis, Joseph G.
In: Brain Research, Vol. 575, No. 2, 20.03.1992, p. 215-222.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Functional neurolobectomy induced by controlled compression of the pituitary stalk
AU - Dohanics, Janos
AU - Hoffman, Gloria E.
AU - Smith, M (Susan)
AU - Verbalis, Joseph G.
PY - 1992/3/20
Y1 - 1992/3/20
N2 - Degeneration of magnocellular nerve terminals in the neurohypophysis was induced by compressing the pituitary stalk of anesthetized rats for 30 s using a triangle-shaped wire. Immediately after stalk compression (SC), rats exhibited markedly increased water intake characteristic of diabetes insipidus, followed by a triphasic pattern of fluid intake. In SC rats, arginine vasopressin (AVP) and oxitocin (OT) contents of the neurointermediate lobe (NIL) of the pituitary gland were significantly reduced to ∼2.5% and ∼10% of sham-operated controls, respectivel In contrast, OT, but not AVP, content of the stalk-median eminence (SME) of SC rats was significantly increased. Histological examination of the pituitaries showed substantial degeneration of the neural lobe with very scarce AVP-neurophysin and OT-neurophysin immunoreactivity, while both the anterior and the intermediate lobes appeared to be intact. Plasma AVP and OT responses to infusion of hypertonic NaCl were significantly blunted in SC rats compared to sham-operated controls. However, two days after surgery the secretory patterns of LH in SC rats were similar to those in the controls. These results indicate that controlled compression of the pituitary stalk results in selective degeneration of the neural lobe without causing permanent ischemic damage to the anterior pituitary, and produces marked sustained functional deficits in pituitary AVP and OT secretion. Consequently, SC provides an alternatiove means to achieve selective neurolobectomy in rats.
AB - Degeneration of magnocellular nerve terminals in the neurohypophysis was induced by compressing the pituitary stalk of anesthetized rats for 30 s using a triangle-shaped wire. Immediately after stalk compression (SC), rats exhibited markedly increased water intake characteristic of diabetes insipidus, followed by a triphasic pattern of fluid intake. In SC rats, arginine vasopressin (AVP) and oxitocin (OT) contents of the neurointermediate lobe (NIL) of the pituitary gland were significantly reduced to ∼2.5% and ∼10% of sham-operated controls, respectivel In contrast, OT, but not AVP, content of the stalk-median eminence (SME) of SC rats was significantly increased. Histological examination of the pituitaries showed substantial degeneration of the neural lobe with very scarce AVP-neurophysin and OT-neurophysin immunoreactivity, while both the anterior and the intermediate lobes appeared to be intact. Plasma AVP and OT responses to infusion of hypertonic NaCl were significantly blunted in SC rats compared to sham-operated controls. However, two days after surgery the secretory patterns of LH in SC rats were similar to those in the controls. These results indicate that controlled compression of the pituitary stalk results in selective degeneration of the neural lobe without causing permanent ischemic damage to the anterior pituitary, and produces marked sustained functional deficits in pituitary AVP and OT secretion. Consequently, SC provides an alternatiove means to achieve selective neurolobectomy in rats.
KW - Adenohypophysis
KW - Neurohypophysis
KW - Oxytocin
KW - Pituitary stalk
KW - Vasopressin
UR - http://www.scopus.com/inward/record.url?scp=0026532053&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026532053&partnerID=8YFLogxK
U2 - 10.1016/0006-8993(92)90082-K
DO - 10.1016/0006-8993(92)90082-K
M3 - Article
C2 - 1571781
AN - SCOPUS:0026532053
VL - 575
SP - 215
EP - 222
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 2
ER -