Functional mechanisms underlying pleiotropic risk alleles at the 19p13.1 breast-ovarian cancer susceptibility locus

KConFab, The Hereditary Breast and Ovarian Cancer Research Group Netherlands (HEBON), EMBRACE, GEMO Study Collaboratorsz, Australian Ovarian Cancer Study Groupz

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A locus at 19p13 is associated with breast cancer (BC) and ovarian cancer (OC) risk. Here we analyse 438 SNPs in this region in 46,451 BC and 15,438 OC cases, 15,252 BRCA1 mutation carriers and 73,444 controls and identify 13 candidate causal SNPs associated with serous OC (P=9.2 × 10-20), ER-negative BC (P=1.1 × 10-13), BRCA1-associated BC (P=7.7 × 10-16) and triple negative BC (P-diff=2 × 10-5). Genotype-gene expression associations are identified for candidate target genes ANKLE1 (P=2 × 10-3) and ABHD8 (P<2 × 10-3). Chromosome conformation capture identifies interactions between four candidate SNPs and ABHD8, and luciferase assays indicate six risk alleles increased transactivation of the ADHD8 promoter. Targeted deletion of a region containing risk SNP rs56069439 in a putative enhancer induces ANKLE1 downregulation; and mRNA stability assays indicate functional effects for an ANKLE1 3′-UTR SNP. Altogether, these data suggest that multiple SNPs at 19p13 regulate ABHD8 and perhaps ANKLE1 expression, and indicate common mechanisms underlying breast and ovarian cancer risk.

Original languageEnglish (US)
Article number12675
JournalNature Communications
Volume7
DOIs
StatePublished - Sep 7 2016

Fingerprint

loci
breast
Ovarian Neoplasms
Single Nucleotide Polymorphism
cancer
Alleles
Breast Neoplasms
magnetic permeability
Assays
3' Untranslated Regions
Chromosomes
Luciferases
Gene expression
Triple Negative Breast Neoplasms
Conformations
Genes
RNA Stability
Messenger RNA
Transcriptional Activation
deletion

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

KConFab, The Hereditary Breast and Ovarian Cancer Research Group Netherlands (HEBON), EMBRACE, GEMO Study Collaboratorsz, & Australian Ovarian Cancer Study Groupz (2016). Functional mechanisms underlying pleiotropic risk alleles at the 19p13.1 breast-ovarian cancer susceptibility locus. Nature Communications, 7, [12675]. https://doi.org/10.1038/ncomms12675

Functional mechanisms underlying pleiotropic risk alleles at the 19p13.1 breast-ovarian cancer susceptibility locus. / KConFab; The Hereditary Breast and Ovarian Cancer Research Group Netherlands (HEBON); EMBRACE; GEMO Study Collaboratorsz; Australian Ovarian Cancer Study Groupz.

In: Nature Communications, Vol. 7, 12675, 07.09.2016.

Research output: Contribution to journalArticle

KConFab, The Hereditary Breast and Ovarian Cancer Research Group Netherlands (HEBON), EMBRACE, GEMO Study Collaboratorsz & Australian Ovarian Cancer Study Groupz 2016, 'Functional mechanisms underlying pleiotropic risk alleles at the 19p13.1 breast-ovarian cancer susceptibility locus', Nature Communications, vol. 7, 12675. https://doi.org/10.1038/ncomms12675
KConFab, The Hereditary Breast and Ovarian Cancer Research Group Netherlands (HEBON), EMBRACE, GEMO Study Collaboratorsz, Australian Ovarian Cancer Study Groupz. Functional mechanisms underlying pleiotropic risk alleles at the 19p13.1 breast-ovarian cancer susceptibility locus. Nature Communications. 2016 Sep 7;7. 12675. https://doi.org/10.1038/ncomms12675
KConFab ; The Hereditary Breast and Ovarian Cancer Research Group Netherlands (HEBON) ; EMBRACE ; GEMO Study Collaboratorsz ; Australian Ovarian Cancer Study Groupz. / Functional mechanisms underlying pleiotropic risk alleles at the 19p13.1 breast-ovarian cancer susceptibility locus. In: Nature Communications. 2016 ; Vol. 7.
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abstract = "A locus at 19p13 is associated with breast cancer (BC) and ovarian cancer (OC) risk. Here we analyse 438 SNPs in this region in 46,451 BC and 15,438 OC cases, 15,252 BRCA1 mutation carriers and 73,444 controls and identify 13 candidate causal SNPs associated with serous OC (P=9.2 × 10-20), ER-negative BC (P=1.1 × 10-13), BRCA1-associated BC (P=7.7 × 10-16) and triple negative BC (P-diff=2 × 10-5). Genotype-gene expression associations are identified for candidate target genes ANKLE1 (P=2 × 10-3) and ABHD8 (P<2 × 10-3). Chromosome conformation capture identifies interactions between four candidate SNPs and ABHD8, and luciferase assays indicate six risk alleles increased transactivation of the ADHD8 promoter. Targeted deletion of a region containing risk SNP rs56069439 in a putative enhancer induces ANKLE1 downregulation; and mRNA stability assays indicate functional effects for an ANKLE1 3′-UTR SNP. Altogether, these data suggest that multiple SNPs at 19p13 regulate ABHD8 and perhaps ANKLE1 expression, and indicate common mechanisms underlying breast and ovarian cancer risk.",
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