Functional integration of adult-born hippocampal neurons after traumatic brain injury

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Traumatic brain injury (TBI) increases hippocampal neurogenesis, which may contribute to cognitive recovery after injury. However, it is unknown whether TBI-induced adult-born neurons mature normally and functionally integrate into the hippocampal network. We assessed the generation, morphology, and synaptic integration of new hippocampal neurons after a controlled cortical impact (CCI) injury model of TBI. To label TBI-induced newborn neurons, we used 2-month-old POMC-EGFP mice, which transiently and specifically express EGFP in immature hippocampal neurons, and doublecortin-CreERT2 transgenic mice crossed with Rosa26-CAGtdTomato reporter mice, to permanently pulse-label a cohort of adult-born hippocampal neurons. TBI increased the generation, outward migration, and dendritic complexity of neurons born during post-traumatic neurogenesis. Cells born after TBI had profound alterations in their dendritic structure, with increased dendritic branching proximal to the soma and widely splayed dendritic branches. These changes were apparent during early dendritic outgrowth and persisted as these cells matured. Whole-cell recordings from neurons generated during posttraumatic neurogenesis demonstrate that they are excitable and functionally integrate into the hippocampal circuit. However, despite their dramatic morphologic abnormalities, we found no differences in the rate of their electrophysiological maturation, or their overall degree of synaptic integration when compared to age-matched adult-born cells from sham mice. Our results suggest that cells born after TBI participate in information processing, and receive an apparently normal balance of excitatory and inhibitory inputs. However, TBI-induced changes in their anatomic localization and dendritic projection patterns could result in maladaptive network properties.

Original languageEnglish (US)
Article numbere0056-15.2015
JournaleNeuro
Volume2
Issue number5
DOIs
StatePublished - Jan 1 2015

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Neurons
Neurogenesis
Pro-Opiomelanocortin
Traumatic Brain Injury
Wounds and Injuries
Carisoprodol
Patch-Clamp Techniques
Automatic Data Processing
Transgenic Mice

Keywords

  • Adult neurogenesis
  • Functional integration
  • Hippocampus
  • Maturation
  • Synaptic integration
  • Traumatic brain injury

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Functional integration of adult-born hippocampal neurons after traumatic brain injury. / Villasana, Laura; Kim, Kristine N.; Westbrook, Gary; Schnell, Eric.

In: eNeuro, Vol. 2, No. 5, e0056-15.2015, 01.01.2015.

Research output: Contribution to journalArticle

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