Functional evidence for an ovarian cancer tumor suppressor gene on chromosome 22 by microcell-mediated chromosome transfer

Russell P. Kruzelock, Bruce D. Cuevas, Jon R. Wiener, Feng Ji Xu, Yinhua Yu, Yofre Cabeza-Arvelaiz, Mark Pershouse, M. Mercedes Lovell, Ann M. Killary, Gordon Mills, Robert C. Bast

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The identity of many tumor suppressor genes important in epithelial ovarian cancer tumorigenesis remains unknown. In an effort to localize a novel tumor suppressor on chromosome 22, a psv2neo tagged human chromosome 22 was transferred into the malignant epithelial ovarian cancer cell line, SKOv-3, by micro-cell-mediated chromosome transfer. Complete suppression of the transformed phenotype was observed in 16 of 18 individual microcell hybrid clones as evidenced by the complete abrogation of cell growth under anchorage-independent conditions. In vitro doubling times were also dramatically reduced, as was the ability to form subcutaneous tumors in CD1 nu/nu mice. Only one polymorphic marker, D22S429, segregated with decreased transformation and tumorigenic potential, suggesting that an unrecognized tumor suppressor may localize to chromosome 22q11-q12. These data provide functional support for the presence of a novel tumor suppressor locus (or loci) on chromosome 22 that is important in ovarian cancer tumorigenesis.

Original languageEnglish (US)
Pages (from-to)6277-6285
Number of pages9
JournalOncogene
Volume19
Issue number54
DOIs
StatePublished - Dec 14 2000
Externally publishedYes

Fingerprint

Chromosomes, Human, Pair 22
Tumor Suppressor Genes
Ovarian Neoplasms
Chromosomes
Neoplasms
Carcinogenesis
Neoplastic Cell Transformation
Human Chromosomes
Clone Cells
Phenotype
Cell Line
Growth
Ovarian epithelial cancer

Keywords

  • Human chromosome 22
  • Microcell-mediated chromosome transfer
  • Ovarian cancer
  • Tumor suppressor genes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Kruzelock, R. P., Cuevas, B. D., Wiener, J. R., Xu, F. J., Yu, Y., Cabeza-Arvelaiz, Y., ... Bast, R. C. (2000). Functional evidence for an ovarian cancer tumor suppressor gene on chromosome 22 by microcell-mediated chromosome transfer. Oncogene, 19(54), 6277-6285. https://doi.org/10.1038/sj.onc.1204013

Functional evidence for an ovarian cancer tumor suppressor gene on chromosome 22 by microcell-mediated chromosome transfer. / Kruzelock, Russell P.; Cuevas, Bruce D.; Wiener, Jon R.; Xu, Feng Ji; Yu, Yinhua; Cabeza-Arvelaiz, Yofre; Pershouse, Mark; Lovell, M. Mercedes; Killary, Ann M.; Mills, Gordon; Bast, Robert C.

In: Oncogene, Vol. 19, No. 54, 14.12.2000, p. 6277-6285.

Research output: Contribution to journalArticle

Kruzelock, RP, Cuevas, BD, Wiener, JR, Xu, FJ, Yu, Y, Cabeza-Arvelaiz, Y, Pershouse, M, Lovell, MM, Killary, AM, Mills, G & Bast, RC 2000, 'Functional evidence for an ovarian cancer tumor suppressor gene on chromosome 22 by microcell-mediated chromosome transfer', Oncogene, vol. 19, no. 54, pp. 6277-6285. https://doi.org/10.1038/sj.onc.1204013
Kruzelock, Russell P. ; Cuevas, Bruce D. ; Wiener, Jon R. ; Xu, Feng Ji ; Yu, Yinhua ; Cabeza-Arvelaiz, Yofre ; Pershouse, Mark ; Lovell, M. Mercedes ; Killary, Ann M. ; Mills, Gordon ; Bast, Robert C. / Functional evidence for an ovarian cancer tumor suppressor gene on chromosome 22 by microcell-mediated chromosome transfer. In: Oncogene. 2000 ; Vol. 19, No. 54. pp. 6277-6285.
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