Functional evidence for an ovarian cancer tumor suppressor gene on chromosome 22 by microcell-mediated chromosome transfer

Russell P. Kruzelock, Bruce D. Cuevas, Jon R. Wiener, Feng Ji Xu, Yinhua Yu, Yofre Cabeza-Arvelaiz, Mark Pershouse, M. Mercedes Lovell, Ann M. Killary, Gordon B. Mills, Robert C. Bast

    Research output: Contribution to journalArticle

    8 Scopus citations

    Abstract

    The identity of many tumor suppressor genes important in epithelial ovarian cancer tumorigenesis remains unknown. In an effort to localize a novel tumor suppressor on chromosome 22, a psv2neo tagged human chromosome 22 was transferred into the malignant epithelial ovarian cancer cell line, SKOv-3, by micro-cell-mediated chromosome transfer. Complete suppression of the transformed phenotype was observed in 16 of 18 individual microcell hybrid clones as evidenced by the complete abrogation of cell growth under anchorage-independent conditions. In vitro doubling times were also dramatically reduced, as was the ability to form subcutaneous tumors in CD1 nu/nu mice. Only one polymorphic marker, D22S429, segregated with decreased transformation and tumorigenic potential, suggesting that an unrecognized tumor suppressor may localize to chromosome 22q11-q12. These data provide functional support for the presence of a novel tumor suppressor locus (or loci) on chromosome 22 that is important in ovarian cancer tumorigenesis.

    Original languageEnglish (US)
    Pages (from-to)6277-6285
    Number of pages9
    JournalOncogene
    Volume19
    Issue number54
    DOIs
    StatePublished - Dec 14 2000

    Keywords

    • Human chromosome 22
    • Microcell-mediated chromosome transfer
    • Ovarian cancer
    • Tumor suppressor genes

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics
    • Cancer Research

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  • Cite this

    Kruzelock, R. P., Cuevas, B. D., Wiener, J. R., Xu, F. J., Yu, Y., Cabeza-Arvelaiz, Y., Pershouse, M., Lovell, M. M., Killary, A. M., Mills, G. B., & Bast, R. C. (2000). Functional evidence for an ovarian cancer tumor suppressor gene on chromosome 22 by microcell-mediated chromosome transfer. Oncogene, 19(54), 6277-6285. https://doi.org/10.1038/sj.onc.1204013