Functional Doppler optical coherence tomography for cortical blood flow imaging

Lingfeng Yu, Gangjun Liu, Elaine Nguyen, Bernard Choi, Zhongping Chen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Optical methods have been widely used in basic neuroscience research to study the cerebral blood flow dynamics in order to overcome the low spatial resolution associated with magnetic resonance imaging and positron emission tomography. Although laser Doppler imaging and laser speckle imaging can map out en face cortical hemodynamics and columns, depth resolution is not available. Two-photon microscopy has been used for mapping cortical activity. However, flow measurement requires fluorescent dye injection, which can be problematic. The noninvasive and high resolution tomographic capabilities of optical coherence tomography make it a promising technique for mapping depth resolved cortical blood flow. Here, we present a functional Doppler optical coherence tomography (OCT) imaging modality for quantitative evaluation of cortical blood flow in a mouse model. Fast, repeated, Doppler OCT scans across a vessel of interest were performed to record flow dynamic information with a high temporal resolution of the cardiac cycles. Spectral Doppler analysis of continuous Doppler images demonstrates how the velocity components and longitudinally projected flow-volume-rate change over time, thereby providing complementary temporal flow information to the spatially distributed flow information of Doppler OCT. The proposed functional Doppler OCT imaging modality can be used to diagnose vessel stenosis/blockage or monitor blood flow changes due to pharmacological agents/neuronal activities. Non-invasive in-vivo mice experiments were performed to verify the capabilities of function Doppler OCT.

Original languageEnglish (US)
Title of host publicationPhotonic Therapeutics and Diagnostics VI
DOIs
StatePublished - Dec 1 2010
EventPhotonic Therapeutics and Diagnostics VI - San Francisco, CA, United States
Duration: Jan 23 2010Jan 25 2010

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7548
ISSN (Print)1605-7422

Other

OtherPhotonic Therapeutics and Diagnostics VI
CountryUnited States
CitySan Francisco, CA
Period1/23/101/25/10

Keywords

  • Doppler
  • functional optical coherence tomography
  • vessel stenosis
  • visualization of neural activity

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Fingerprint Dive into the research topics of 'Functional Doppler optical coherence tomography for cortical blood flow imaging'. Together they form a unique fingerprint.

  • Cite this

    Yu, L., Liu, G., Nguyen, E., Choi, B., & Chen, Z. (2010). Functional Doppler optical coherence tomography for cortical blood flow imaging. In Photonic Therapeutics and Diagnostics VI [75483Q] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7548). https://doi.org/10.1117/12.842820