Functional coupling between neurons and glia

Veronica Alvarez-Maubecin, Fernando García-Hernández, John Williams, Elisabeth J. Van Bockstaele

Research output: Contribution to journalArticle

261 Citations (Scopus)

Abstract

Neuronal-glial interactions play an important role in information processing in the CNS. Previous studies have indicated that electrotonic coupling between locus ceruleus (LC) neurons is involved in synchronizing the spontaneous activity. The results of the present study extend the functional electrotonic coupling to interactions between neurons and glia. Spontaneous oscillations in the membrane potential were observed in a subset of glia. These oscillations were synchronous with the firing of neurons, insensitive to transmitter receptor antagonists and disrupted by carbenoxolone, a gap junction blocker. Hyperpolarization of neurons with [Met] 5enkephalin blocked the oscillations in gila. Selective depolarization of glia with a glutamate transporter substrate (L-α-aminoadipic acid) increased the neuronal firing rate, suggesting that changes in the membrane potential of glia can modulate neuronal excitability through heterocellular coupling. Dye- coupling experiments further confirmed that small molecules could be transferred through gap junctions between these distinct cell types. No dye transfer was observed between neurons and oligodendrocytes or between astrocytes and oligodendrocytes, suggesting that the junctional communication was specific for astrocytes and neurons. Finally, immunoelectron microscopy studies established that connexins, the proteins that form gap junctions, were present on portions of the plasmalemma, bridging the cytoplasm of neurons and glia in LC. This heterocellular coupling extends the mechanisms by which glia participate in the network properties of the LC in which the degree of coupling is thought to influence cognitive performance.

Original languageEnglish (US)
Pages (from-to)4091-4098
Number of pages8
JournalJournal of Neuroscience
Volume20
Issue number11
StatePublished - Jun 1 2000

Fingerprint

Neuroglia
Neurons
Locus Coeruleus
Connexins
Gap Junctions
Oligodendroglia
Astrocytes
Membrane Potentials
Coloring Agents
Carbenoxolone
Amino Acid Transport System X-AG
Immunoelectron Microscopy
Automatic Data Processing
Cytoplasm
Acids

Keywords

  • Connexins
  • Electrotonic coupling
  • Gap junction
  • Membrane potential oscillations
  • Neuronal-glial interactions
  • Noradrenergic neurons

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Alvarez-Maubecin, V., García-Hernández, F., Williams, J., & Van Bockstaele, E. J. (2000). Functional coupling between neurons and glia. Journal of Neuroscience, 20(11), 4091-4098.

Functional coupling between neurons and glia. / Alvarez-Maubecin, Veronica; García-Hernández, Fernando; Williams, John; Van Bockstaele, Elisabeth J.

In: Journal of Neuroscience, Vol. 20, No. 11, 01.06.2000, p. 4091-4098.

Research output: Contribution to journalArticle

Alvarez-Maubecin, V, García-Hernández, F, Williams, J & Van Bockstaele, EJ 2000, 'Functional coupling between neurons and glia', Journal of Neuroscience, vol. 20, no. 11, pp. 4091-4098.
Alvarez-Maubecin V, García-Hernández F, Williams J, Van Bockstaele EJ. Functional coupling between neurons and glia. Journal of Neuroscience. 2000 Jun 1;20(11):4091-4098.
Alvarez-Maubecin, Veronica ; García-Hernández, Fernando ; Williams, John ; Van Bockstaele, Elisabeth J. / Functional coupling between neurons and glia. In: Journal of Neuroscience. 2000 ; Vol. 20, No. 11. pp. 4091-4098.
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