Functional complementation by electroporation of human BACs into mammalian fibroblast cells

J. A. Hejna, P. L. Johnstone, S. L. Kohler, D. A. Bruun, C. A. Reifsteck, Susan Olson, Robb Moses

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Bacterial Artificial Chromosomes (BACs) have been used to complement a metabolic defect and to transfer a drug resistance marker into mammalian cells by electroporation. The selectable markers are stable and the recipient cells have BAC DNA integrated into the chromosomes as shown by fluorescent in situ hybridization, PCR and Southern hybridization.

Original languageEnglish (US)
Pages (from-to)1124-1125
Number of pages2
JournalNucleic Acids Research
Volume26
Issue number4
DOIs
StatePublished - Feb 15 1998

Fingerprint

Human Artificial Chromosomes
Bacterial Artificial Chromosomes
Electroporation
Fibroblasts
Fluorescence In Situ Hybridization
Drug Resistance
Chromosomes
Polymerase Chain Reaction
DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Functional complementation by electroporation of human BACs into mammalian fibroblast cells. / Hejna, J. A.; Johnstone, P. L.; Kohler, S. L.; Bruun, D. A.; Reifsteck, C. A.; Olson, Susan; Moses, Robb.

In: Nucleic Acids Research, Vol. 26, No. 4, 15.02.1998, p. 1124-1125.

Research output: Contribution to journalArticle

Hejna, J. A. ; Johnstone, P. L. ; Kohler, S. L. ; Bruun, D. A. ; Reifsteck, C. A. ; Olson, Susan ; Moses, Robb. / Functional complementation by electroporation of human BACs into mammalian fibroblast cells. In: Nucleic Acids Research. 1998 ; Vol. 26, No. 4. pp. 1124-1125.
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