Functional complementation by electroporation of human BACs into mammalian fibroblast cells

J. A. Hejna, P. L. Johnstone, S. L. Kohler, D. A. Bruun, C. A. Reifsteck, S. B. Olson, R. E. Moses

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Bacterial Artificial Chromosomes (BACs) have been used to complement a metabolic defect and to transfer a drug resistance marker into mammalian cells by electroporation. The selectable markers are stable and the recipient cells have BAC DNA integrated into the chromosomes as shown by fluorescent in situ hybridization, PCR and Southern hybridization.

Original languageEnglish (US)
Pages (from-to)1124-1125
Number of pages2
JournalNucleic acids research
Volume26
Issue number4
DOIs
StatePublished - Feb 15 1998

ASJC Scopus subject areas

  • Genetics

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