Functional complementation by electroporation of human BACs into mammalian fibroblast cells

J. A. Hejna; P. L. Johnstone; S. L. Kohler; D. A. Bruun; C. A. Reifsteck; S. B. Olson; R. E. Moses

doi:10.1093/nar/26.4.1124

Functional complementation by electroporation of human BACs into mammalian fibroblast cells

J. A. Hejna, P. L. Johnstone, S. L. Kohler, D. A. Bruun, C. A. Reifsteck, S. B. Olson, R. E. Moses

Molecular and Medical Genetics

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Bacterial Artificial Chromosomes (BACs) have been used to complement a metabolic defect and to transfer a drug resistance marker into mammalian cells by electroporation. The selectable markers are stable and the recipient cells have BAC DNA integrated into the chromosomes as shown by fluorescent in situ hybridization, PCR and Southern hybridization.

Original language	English (US)
Pages (from-to)	1124-1125
Number of pages	2
Journal	Nucleic acids research
Volume	26
Issue number	4
DOIs	https://doi.org/10.1093/nar/26.4.1124
State	Published - Feb 15 1998

ASJC Scopus subject areas

Genetics

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title = "Functional complementation by electroporation of human BACs into mammalian fibroblast cells",

abstract = "Bacterial Artificial Chromosomes (BACs) have been used to complement a metabolic defect and to transfer a drug resistance marker into mammalian cells by electroporation. The selectable markers are stable and the recipient cells have BAC DNA integrated into the chromosomes as shown by fluorescent in situ hybridization, PCR and Southern hybridization.",

author = "Hejna, {J. A.} and Johnstone, {P. L.} and Kohler, {S. L.} and Bruun, {D. A.} and Reifsteck, {C. A.} and Olson, {S. B.} and Moses, {R. E.}",

note = "Funding Information: This research supported by a grant from the National Institute of Health (1P01HL48546).",

year = "1998",

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AU - Hejna, J. A.

AU - Johnstone, P. L.

AU - Kohler, S. L.

AU - Bruun, D. A.

AU - Reifsteck, C. A.

AU - Olson, S. B.

AU - Moses, R. E.

N1 - Funding Information: This research supported by a grant from the National Institute of Health (1P01HL48546).

PY - 1998/2/15

Y1 - 1998/2/15

N2 - Bacterial Artificial Chromosomes (BACs) have been used to complement a metabolic defect and to transfer a drug resistance marker into mammalian cells by electroporation. The selectable markers are stable and the recipient cells have BAC DNA integrated into the chromosomes as shown by fluorescent in situ hybridization, PCR and Southern hybridization.

AB - Bacterial Artificial Chromosomes (BACs) have been used to complement a metabolic defect and to transfer a drug resistance marker into mammalian cells by electroporation. The selectable markers are stable and the recipient cells have BAC DNA integrated into the chromosomes as shown by fluorescent in situ hybridization, PCR and Southern hybridization.

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Functional complementation by electroporation of human BACs into mammalian fibroblast cells

Abstract

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