Abstract
Recombinant vaccinia viruses harboring the human dopamine D4 receptor cDNA containing two 48 base pair-repeats (D4.2) or the rat dopamine D2 short (D2s) receptor cDNA were used to infect rat-1 fibroblasts. Heterologous expression of both dopamine receptors was demonstrated in binding assays. The affinity constants of these receptors were consistent with values previously reported, including D4.2's higher affinity for the antipsychotic clozapine and raclopride's selectivity for D2 receptors. In the presence of 200 μM 5′-guanylyl- imidodiphosphate (Gpp[NH]p) both receptors exhibited reduced affinities for dopamine. Furthermore, when rat-1 cells were infected with the D2s or the D4.2 recombinant vaccinia viruses and exposed to dopamine agonists, the inhibition of adenylyl cyclase activity was prevented in pertussis toxin-treated cells. This study demonstrates the utility of recombinant receptor-vaccinia viruses in studies of expression, pharmacology and functional coupling of inhibitory G protein-coupled receptors.
Original language | English (US) |
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Pages (from-to) | 11-17 |
Number of pages | 7 |
Journal | European Journal of Pharmacology: Molecular Pharmacology |
Volume | 290 |
Issue number | 1 |
DOIs | |
State | Published - Jun 23 1995 |
Keywords
- (Functional expression)
- Adenylyl cyclase
- Dopamine D receptor
- Vaccinia virus
ASJC Scopus subject areas
- Pharmacology