Functional characterization of the human dopamine D4.2 receptor using vaccinia virus as an expression system

Claudia Bouvier, James R. Bunzow, Hong Chang Guan, Anja Unteutsch, Olivier Civelli, David Grandy, Hubert H M Van Tol

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    Recombinant vaccinia viruses harboring the human dopamine D4 receptor cDNA containing two 48 base pair-repeats (D4.2) or the rat dopamine D2 short (D2s) receptor cDNA were used to infect rat-1 fibroblasts. Heterologous expression of both dopamine receptors was demonstrated in binding assays. The affinity constants of these receptors were consistent with values previously reported, including D4.2's higher affinity for the antipsychotic clozapine and raclopride's selectivity for D2 receptors. In the presence of 200 μM 5′-guanylyl- imidodiphosphate (Gpp[NH]p) both receptors exhibited reduced affinities for dopamine. Furthermore, when rat-1 cells were infected with the D2s or the D4.2 recombinant vaccinia viruses and exposed to dopamine agonists, the inhibition of adenylyl cyclase activity was prevented in pertussis toxin-treated cells. This study demonstrates the utility of recombinant receptor-vaccinia viruses in studies of expression, pharmacology and functional coupling of inhibitory G protein-coupled receptors.

    Original languageEnglish (US)
    Pages (from-to)11-17
    Number of pages7
    JournalEuropean Journal of Pharmacology: Molecular Pharmacology
    Volume290
    Issue number1
    DOIs
    StatePublished - Jun 23 1995

    Fingerprint

    Vaccinia virus
    Dopamine Receptors
    Guanylyl Imidodiphosphate
    Complementary DNA
    Dopamine D4 Receptors
    Raclopride
    Dopamine D2 Receptors
    Clozapine
    Dopamine Agonists
    Pertussis Toxin
    G-Protein-Coupled Receptors
    Adenylyl Cyclases
    Base Pairing
    Antipsychotic Agents
    Dopamine
    Fibroblasts
    Pharmacology

    Keywords

    • (Functional expression)
    • Adenylyl cyclase
    • Dopamine D receptor
    • Vaccinia virus

    ASJC Scopus subject areas

    • Pharmacology

    Cite this

    Functional characterization of the human dopamine D4.2 receptor using vaccinia virus as an expression system. / Bouvier, Claudia; Bunzow, James R.; Guan, Hong Chang; Unteutsch, Anja; Civelli, Olivier; Grandy, David; Van Tol, Hubert H M.

    In: European Journal of Pharmacology: Molecular Pharmacology, Vol. 290, No. 1, 23.06.1995, p. 11-17.

    Research output: Contribution to journalArticle

    Bouvier, Claudia ; Bunzow, James R. ; Guan, Hong Chang ; Unteutsch, Anja ; Civelli, Olivier ; Grandy, David ; Van Tol, Hubert H M. / Functional characterization of the human dopamine D4.2 receptor using vaccinia virus as an expression system. In: European Journal of Pharmacology: Molecular Pharmacology. 1995 ; Vol. 290, No. 1. pp. 11-17.
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