Functional characterization of cell-wall-associated protein WapA in Streptococcus mutans

Lin Zhu, Jens Kreth, Sarah E. Cross, James K. Gimzewski, Wenyuan Shi, Fengxia Qi

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Streptococcus mutans is known as a primary pathogen responsible for dental caries. One of the virulence factors of S. mutans in cariogenicity is its ability to attach to the tooth surface and form a biofilm. Several surface proteins have been shown to be involved in this process. A 29 kDa surface protein named wall-associated protein A (WapA, antigen A or antigen III), was previously used as a vaccine in animal studies for immunization against dental caries. However, the function of WapA in S. mutans is still not clear. This study characterized the function of WapA in cell surface structure and biofilm formation. Compared to the wild-type, the wapA mutant had much-reduced cell chain length, diminished cell-cell aggregation, altered cell surface ultrastructure, and unstructured biofilm architecture. Furthermore, in vivo force spectroscopy revealed that the cell surface of the wapA mutant was less sticky than that of the wild-type cells. More interestingly, these phenotypic differences diminished as sucrose concentration in the medium was increased to 0.5 %. Real-time RT-PCR analysis demonstrated that sucrose strongly repressed wapA gene expression in both planktonic and biofilm cells. These results suggest that the WapA protein plays an important structural role on the cell surface, which ultimately affects sucrose-independent cell-cell aggregation and biofilm architecture.

Original languageEnglish (US)
Pages (from-to)2395-2404
Number of pages10
JournalMicrobiology
Volume152
Issue number8
DOIs
StatePublished - Aug 1 2006
Externally publishedYes

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Streptococcus mutans
Cell Wall
Biofilms
Proteins
Sucrose
Cell Aggregation
Dental Caries
Membrane Proteins
Antigens
Staphylococcal Protein A
Virulence Factors
Real-Time Polymerase Chain Reaction
Immunization
Spectrum Analysis
Tooth
Vaccines
Gene Expression

ASJC Scopus subject areas

  • Microbiology

Cite this

Functional characterization of cell-wall-associated protein WapA in Streptococcus mutans. / Zhu, Lin; Kreth, Jens; Cross, Sarah E.; Gimzewski, James K.; Shi, Wenyuan; Qi, Fengxia.

In: Microbiology, Vol. 152, No. 8, 01.08.2006, p. 2395-2404.

Research output: Contribution to journalArticle

Zhu, Lin ; Kreth, Jens ; Cross, Sarah E. ; Gimzewski, James K. ; Shi, Wenyuan ; Qi, Fengxia. / Functional characterization of cell-wall-associated protein WapA in Streptococcus mutans. In: Microbiology. 2006 ; Vol. 152, No. 8. pp. 2395-2404.
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