Function and innervation of the locus ceruleus in a macaque model of Functional Hypothalamic Amenorrhea

Cynthia Bethea, Aaron Kim, Judy L. Cameron

    Research output: Contribution to journalArticle

    10 Citations (Scopus)

    Abstract

    A body of knowledge implicates an increase in output from the locus ceruleus (LC) during stress. We questioned the innervation and function of the LC in our macaque model of Functional Hypothalamic Amenorrhea, also known as Stress-Induced Amenorrhea. Cohorts of macaques were initially characterized as highly stress resilient (HSR) or stress-sensitive (SS) based upon the presence or absence of ovulation during a protocol involving 2 menstrual cycles with psychosocial and metabolic stress. Afterwards, the animals were rested until normal menstrual cycles resumed and then euthanized on day 5 of a new menstrual cycle [a] in the absence of further stress; or [b] after 5. days of resumed psychosocial and metabolic stress. In this study, parameters of the LC were examined in HSR and SS animals in the presence and absence of stress (2. ×. 2 block design) using ICC and image analysis. Tyrosine hydroxylase (TH) is the rate-limiting enzyme for the synthesis of catecholamines; and the TH level was used to assess by inference, NE output. The pixel area of TH-positive dendrites extending outside the medial border of the LC was significantly increased by stress to a similar degree in both HSR and SS animals (p.

    Original languageEnglish (US)
    Pages (from-to)96-106
    Number of pages11
    JournalNeurobiology of Disease
    Volume50
    Issue number1
    DOIs
    StatePublished - Feb 2013

    Fingerprint

    Locus Coeruleus
    Amenorrhea
    Macaca
    Tyrosine 3-Monooxygenase
    Menstrual Cycle
    Physiological Stress
    Dendrites
    Ovulation
    Catecholamines
    Enzymes

    Keywords

    • Amenorrhea
    • CRF
    • Dendrites
    • Excitotoxicity
    • Locus ceruleus
    • Macaques
    • Ovulation
    • Serotonin
    • Stress
    • Tyrosine hydroxylase

    ASJC Scopus subject areas

    • Neurology

    Cite this

    Function and innervation of the locus ceruleus in a macaque model of Functional Hypothalamic Amenorrhea. / Bethea, Cynthia; Kim, Aaron; Cameron, Judy L.

    In: Neurobiology of Disease, Vol. 50, No. 1, 02.2013, p. 96-106.

    Research output: Contribution to journalArticle

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