Full-Length P2X7 Structures Reveal How Palmitoylation Prevents Channel Desensitization

Alanna E. McCarthy, Craig Yoshioka, Steven E. Mansoor

Research output: Contribution to journalArticle

Abstract

P2X receptors are trimeric, non-selective cation channels activated by extracellular ATP. The P2X7 receptor subtype is a pharmacological target because of involvement in apoptotic, inflammatory, and tumor progression pathways. It is the most structurally and functionally distinct P2X subtype, containing a unique cytoplasmic domain critical for the receptor to initiate apoptosis and not undergo desensitization. However, lack of structural information about the cytoplasmic domain has hindered understanding of the molecular mechanisms underlying these processes. We report cryoelectron microscopy structures of full-length rat P2X7 receptor in apo and ATP-bound states. These structures reveal how one cytoplasmic element, the C-cys anchor, prevents desensitization by anchoring the pore-lining helix to the membrane with palmitoyl groups. They show a second cytoplasmic element with a unique fold, the cytoplasmic ballast, which unexpectedly contains a zinc ion complex and a guanosine nucleotide binding site. Our structures provide first insights into the architecture and function of a P2X receptor cytoplasmic domain.

Original languageEnglish (US)
Pages (from-to)659-670.e13
JournalCell
Volume179
Issue number3
DOIs
StatePublished - Oct 17 2019

Fingerprint

Purinergic P2X7 Receptors
Lipoylation
Adenosine Triphosphate
Cryoelectron Microscopy
Guanosine
Cytoplasmic and Nuclear Receptors
Anchors
Linings
Cations
Zinc
Rats
Tumors
Microscopic examination
Nucleotides
Binding Sites
Pharmacology
Ions
Apoptosis
Membranes
Neoplasms

Keywords

  • Apoptosis
  • Cryoelectron microscopy
  • Cytoplasmic domain
  • Desensitization
  • Guanosine nucleotides
  • Ligand gated ion channels
  • Palmitoylation
  • Purinergic (P2X) receptors
  • Structural biology
  • Zinc ion complex

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Full-Length P2X7 Structures Reveal How Palmitoylation Prevents Channel Desensitization. / McCarthy, Alanna E.; Yoshioka, Craig; Mansoor, Steven E.

In: Cell, Vol. 179, No. 3, 17.10.2019, p. 659-670.e13.

Research output: Contribution to journalArticle

McCarthy, Alanna E. ; Yoshioka, Craig ; Mansoor, Steven E. / Full-Length P2X7 Structures Reveal How Palmitoylation Prevents Channel Desensitization. In: Cell. 2019 ; Vol. 179, No. 3. pp. 659-670.e13.
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