Frequent aneuploidy among normal human hepatocytes

Andrew W. Duncan, Amy E. Hanlon Newell, Leslie Smith, Elizabeth M. Wilson, Susan B. Olson, Matthew J. Thayer, Stephen C. Strom, Markus Grompe

Research output: Contribution to journalArticle

115 Scopus citations

Abstract

Murine hepatocytes become polyploid and then undergo ploidy reversal and become aneuploid in a dynamic process called the ploidy conveyor. Although polyploidization occurs in some types of human cells, the degree of aneuploidy in human hepatocytes is not known. We isolated hepatocytes derived from healthy human liver samples and determined chromosome number and identity using traditional karyotyping and fluorescence in situ hybridization. Similar to murine hepatocytes, human hepatocytes are highly aneuploid. Moreover, imaging studies revealed multipolar spindles and chromosome segregation defects in dividing human hepatocytes. Aneuploidy therefore does not necessarily predispose liver cells to transformation but might promote genetic diversity among hepatocytes.

Original languageEnglish (US)
Pages (from-to)25-28
Number of pages4
JournalGastroenterology
Volume142
Issue number1
DOIs
StatePublished - Jan 2012

Keywords

  • Chromosomal Instability
  • Lagging Chromosome
  • Liver Regeneration
  • Mitosis

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

Fingerprint Dive into the research topics of 'Frequent aneuploidy among normal human hepatocytes'. Together they form a unique fingerprint.

  • Cite this

    Duncan, A. W., Hanlon Newell, A. E., Smith, L., Wilson, E. M., Olson, S. B., Thayer, M. J., Strom, S. C., & Grompe, M. (2012). Frequent aneuploidy among normal human hepatocytes. Gastroenterology, 142(1), 25-28. https://doi.org/10.1053/j.gastro.2011.10.029