TY - JOUR
T1 - Frequency of T cells specific for myelin basic protein and myelin proteolipid protein in blood and cerebrospinal fluid in multiple sclerosis
AU - Chou, Yuan K.
AU - Bourdette, Dennis N.
AU - Offner, Halina
AU - Whitham, Ruth
AU - Wang, Run Ying
AU - Hashim, George A.
AU - Vandenbark, Arthur A.
N1 - Funding Information:
for their assistance in the precursor frequency analysis and Joe Turner for graphic design. This work was supported by the Department of Veterans Affairs, NIH Grants NS23221, NS23444 and NS21466, the Medical Research Foundation of Oregon and XOMA Corporation.
PY - 1992/5
Y1 - 1992/5
N2 - T cell sensitization to two myelin components, myelin basic protein (MBP) and myelin proteolipid protein (PLP), may be important to the pathogenesis of multiple sclerosis (MS). Using the limiting dilution assay, we demonstrated that the blood of MS patients had an increased frequency of MBP-reactive T cells compared with normal subjects and patients with other neurological diseases (OND) and rheumatoid arthritis. There was no difference in T cell frequency to a synthetic peptide, PLP139-151, or Herpes simplex virus. Within cerebrospinal fluid (CSF), 37% of IL-2/IL-4-reactive T cell isolates from MS patients responded either to MBP or PLP139-151 while only 5% of similar isolates from OND patients responded to these myelin antigens. The mean relative frequency of MBP-reactive T cells within CSF from MS patients was significantly higher than that of OND patients (22 × 10-5 cell versus 1 × 10-5 cells) and was similar to that of MBP reactive T cells within the central nervous system of rats with experimental autoimmune encephalomyelitis. These results lend new support to the hypothesis that myelin-reactive T cells mediate disease in MS.
AB - T cell sensitization to two myelin components, myelin basic protein (MBP) and myelin proteolipid protein (PLP), may be important to the pathogenesis of multiple sclerosis (MS). Using the limiting dilution assay, we demonstrated that the blood of MS patients had an increased frequency of MBP-reactive T cells compared with normal subjects and patients with other neurological diseases (OND) and rheumatoid arthritis. There was no difference in T cell frequency to a synthetic peptide, PLP139-151, or Herpes simplex virus. Within cerebrospinal fluid (CSF), 37% of IL-2/IL-4-reactive T cell isolates from MS patients responded either to MBP or PLP139-151 while only 5% of similar isolates from OND patients responded to these myelin antigens. The mean relative frequency of MBP-reactive T cells within CSF from MS patients was significantly higher than that of OND patients (22 × 10-5 cell versus 1 × 10-5 cells) and was similar to that of MBP reactive T cells within the central nervous system of rats with experimental autoimmune encephalomyelitis. These results lend new support to the hypothesis that myelin-reactive T cells mediate disease in MS.
KW - Multiple sclerosis
KW - Myelin basic protein
KW - Myelin proteolipid protein
KW - T cell frequency
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U2 - 10.1016/0165-5728(92)90095-3
DO - 10.1016/0165-5728(92)90095-3
M3 - Article
C2 - 1374422
AN - SCOPUS:0026556063
SN - 0165-5728
VL - 38
SP - 105
EP - 113
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
IS - 1-2
ER -