Frequency of intrachromosomal homologous recombination induced by UV radiation in normally repairing and excision repair-deficient human cells

Tohru Tsujimura, Veronica M. Maher, Alan R. Godwin, R. Michael Llskay, J. Justin McCormick

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

To investigate the role of DNA damage and nucleotide excision repair in intrachromosomal homologous recombination, a plasmid containing duplicated copies of the gene coding for hygromycin resistance was introduced into the genome of a repair-proficient human cell line, KMST-6, and two repair-deficient lines, XP2OS(SV) from xeroderma pigmentosum complementation group A and XP2YO(SV) from complementation group F. Neither hygromycin-resistance gene codes for a functional enzyme because each contains an insertion/deletion mutation at a unique site, but recombination between the two defective genes can yield hygromycinresistant cells. The rates of spontaneous recombination in normal and xeroderma pigmentosum cell strains containing the recombination substrate were found to be similar. The frequency of UV-induced recombination was determined for three of these cell strains. At low doses, the group A cell strain and the group F cell strain showed a significant increase in frequency of recombinants. The repair-proficient cell strain required 10- to 20-fold higher doses of UV to exhibit comparable increases in frequency of recombinants. These results suggest that unexcised DNA damage, rather than the excision repair process per se, stimulates such recombination.

Original languageEnglish (US)
Pages (from-to)1566-1570
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number4
DOIs
StatePublished - 1990

Keywords

  • Gene conversion
  • Hygromycin resistance
  • Rate of spontaneous recombination
  • Xeroderma pigmentosum

ASJC Scopus subject areas

  • General

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