TY - JOUR
T1 - Frequency distribution of cytomegalovirus envelope glycoprotein genotypes in bone marrow transplant recipients
AU - Fries, Bettina C.
AU - Chon, Snnwen
AU - Boeckh, Michael
AU - Torok-Storb, Beverly
N1 - Funding Information:
Received 26 July 1993: revised 22 November 1993. Presented in part: American Society of Hematology meeting. Anaheim. California. December 1992. All patients gave informed consent in accordance with human experimentation guidelines ofthe US Department of Health and Human Services and the Institutional Review Board of the Fred Hutchinson Cancer Research Center (FHCRC). Patient data and clinical specimens were collected according to guidelines established by the Internal Review Board of the FHCRC. Grant support: National Institutes ofHealth (CA-18029. DK-34431. CA-18221. CA-15704. and HL-36444) and Deutsche Forschungsgemeinschaft (B.C.F.). Reprints or correspondence: Dr. Beverly Torok-Storb, Fred Hutchinson Cancer Research Center. 1124 Columbia st.. M318. Seattle. WA 98104. * Present affiliation: Montefiore Hospital, Bronx. New York.
PY - 1994/4
Y1 - 1994/4
N2 - Using restriction analysis of polymerase chain reaction (PCR)-amplified DNA, the cytomegalovirus (CMV) envelope glycoprotein (gB and gH) genotypes were determined for virus isolates from 128 bone marrow transplant recipients with fatal or nonfatal CMV. All isolates could be assigned to one of four gB and gH genotypes previously identified by DNA sequencing studies. Isolates of gB type 1 were more commonly found to be of gH type 2, whereas gB types 2-4 were more commonly linked to gH type 1. A small frequency of recombination with gB was detected by restriction analysis of DNA from variable regions of the gp55 and gp116 domains. Multiple isolates from various sites of 29 patients were typed and, with three exceptions, the gB genotype remained constant in all isolates from a single patient. Patients who survived CMV infection more commonly shed virus of gB type 1 than those who died (P =.003). This significant difference of gB types among patient subsets is unexplained but raises the possibility that gB genotypes may serve as a marker for pathogenicity of CMV strains in marrow transplant patients.
AB - Using restriction analysis of polymerase chain reaction (PCR)-amplified DNA, the cytomegalovirus (CMV) envelope glycoprotein (gB and gH) genotypes were determined for virus isolates from 128 bone marrow transplant recipients with fatal or nonfatal CMV. All isolates could be assigned to one of four gB and gH genotypes previously identified by DNA sequencing studies. Isolates of gB type 1 were more commonly found to be of gH type 2, whereas gB types 2-4 were more commonly linked to gH type 1. A small frequency of recombination with gB was detected by restriction analysis of DNA from variable regions of the gp55 and gp116 domains. Multiple isolates from various sites of 29 patients were typed and, with three exceptions, the gB genotype remained constant in all isolates from a single patient. Patients who survived CMV infection more commonly shed virus of gB type 1 than those who died (P =.003). This significant difference of gB types among patient subsets is unexplained but raises the possibility that gB genotypes may serve as a marker for pathogenicity of CMV strains in marrow transplant patients.
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U2 - 10.1093/infdis/169.4.769
DO - 10.1093/infdis/169.4.769
M3 - Article
C2 - 8133090
AN - SCOPUS:0028261846
VL - 169
SP - 769
EP - 774
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 4
ER -