Abstract
Using restriction analysis of polymerase chain reaction (PCR)-amplified DNA, the cytomegalovirus (CMV) envelope glycoprotein (gB and gH) genotypes were determined for virus isolates from 128 bone marrow transplant recipients with fatal or nonfatal CMV. All isolates could be assigned to one of four gB and gH genotypes previously identified by DNA sequencing studies. Isolates of gB type 1 were more commonly found to be of gH type 2, whereas gB types 2-4 were more commonly linked to gH type 1. A small frequency of recombination with gB was detected by restriction analysis of DNA from variable regions of the gp55 and gp116 domains. Multiple isolates from various sites of 29 patients were typed and, with three exceptions, the gB genotype remained constant in all isolates from a single patient. Patients who survived CMV infection more commonly shed virus of gB type 1 than those who died (P = .003). This significant difference of gB types among patient subsets is unexplained but raises the possibility that gB genotypes may serve as a marker for pathogenicity of CMV strains in marrow transplant patients.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 769-774 |
| Number of pages | 6 |
| Journal | Journal of Infectious Diseases |
| Volume | 169 |
| Issue number | 4 |
| State | Published - Apr 1994 |
Fingerprint
ASJC Scopus subject areas
- Immunology
- Public Health, Environmental and Occupational Health
Cite this
Frequency distribution of cytomegalovirus envelope glycoprotein genotypes in bone marrow transplant recipients. / Fries, Bettina C.; Chou, Sunwen; Boeckh, Michael; Torok-Storb, Beverly.
In: Journal of Infectious Diseases, Vol. 169, No. 4, 04.1994, p. 769-774.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Frequency distribution of cytomegalovirus envelope glycoprotein genotypes in bone marrow transplant recipients
AU - Fries, Bettina C.
AU - Chou, Sunwen
AU - Boeckh, Michael
AU - Torok-Storb, Beverly
PY - 1994/4
Y1 - 1994/4
N2 - Using restriction analysis of polymerase chain reaction (PCR)-amplified DNA, the cytomegalovirus (CMV) envelope glycoprotein (gB and gH) genotypes were determined for virus isolates from 128 bone marrow transplant recipients with fatal or nonfatal CMV. All isolates could be assigned to one of four gB and gH genotypes previously identified by DNA sequencing studies. Isolates of gB type 1 were more commonly found to be of gH type 2, whereas gB types 2-4 were more commonly linked to gH type 1. A small frequency of recombination with gB was detected by restriction analysis of DNA from variable regions of the gp55 and gp116 domains. Multiple isolates from various sites of 29 patients were typed and, with three exceptions, the gB genotype remained constant in all isolates from a single patient. Patients who survived CMV infection more commonly shed virus of gB type 1 than those who died (P = .003). This significant difference of gB types among patient subsets is unexplained but raises the possibility that gB genotypes may serve as a marker for pathogenicity of CMV strains in marrow transplant patients.
AB - Using restriction analysis of polymerase chain reaction (PCR)-amplified DNA, the cytomegalovirus (CMV) envelope glycoprotein (gB and gH) genotypes were determined for virus isolates from 128 bone marrow transplant recipients with fatal or nonfatal CMV. All isolates could be assigned to one of four gB and gH genotypes previously identified by DNA sequencing studies. Isolates of gB type 1 were more commonly found to be of gH type 2, whereas gB types 2-4 were more commonly linked to gH type 1. A small frequency of recombination with gB was detected by restriction analysis of DNA from variable regions of the gp55 and gp116 domains. Multiple isolates from various sites of 29 patients were typed and, with three exceptions, the gB genotype remained constant in all isolates from a single patient. Patients who survived CMV infection more commonly shed virus of gB type 1 than those who died (P = .003). This significant difference of gB types among patient subsets is unexplained but raises the possibility that gB genotypes may serve as a marker for pathogenicity of CMV strains in marrow transplant patients.
UR - http://www.scopus.com/inward/record.url?scp=0028261846&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028261846&partnerID=8YFLogxK
M3 - Article
C2 - 8133090
AN - SCOPUS:0028261846
VL - 169
SP - 769
EP - 774
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 4
ER -