Fractalkine preferentially mediates arrest and migration of CD16+ monocytes

Petronela Ancuta, Ravi Rao, Ashlee Moses, Andrew Mehle, Sunil K. Shaw, F. William Luscinskas, Dana Gabuzda

Research output: Contribution to journalArticle

389 Citations (Scopus)

Abstract

CD16+ monocytes represent 5-10% of peripheral blood monocytes in normal individuals and are dramatically expanded in several pathological conditions including sepsis, human immunodeficiency virus 1 infection, and cancer. CD16+ monocytes produce high levels of proinflammatory cytokines and may represent dendritic cell precursors in vivo. The mechanisms that mediate the recruitment of CD16+ monocytes into tissues remain unknown. Here we investigate molecular mechanisms of CD16+ monocyte trafficking and show that migration of CD16+ and CD16- monocytes is mediated by distinct combinations of adhesion molecules and chemokine receptors. In contrast to CD16- monocytes, CD16+ monocytes expressed high CX3CR1 and CXCR4 but low CCR2 and CD62L levels and underwent efficient transendothelial migration in response to fractalkine (FKN; FKN/CX3CL1) and stromal-derived factor 1α (CXCL12) but not monocyte chemoattractant protein 1 (CCL2). CD16+ monocytes arrested on cell surface-expressed FKN under flow with higher frequency compared with CD16- monocytes. These results demonstrate that FKN preferentially mediates arrest and migration of CD16+ monocytes and suggest that recruitment of this proinflammatory monocyte subset to vessel walls via the CX3CR1-FKN pathway may contribute to vascular and tissue injury during pathological conditions.

Original languageEnglish (US)
Pages (from-to)1701-1707
Number of pages7
JournalJournal of Experimental Medicine
Volume197
Issue number12
DOIs
StatePublished - Jun 16 2003

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Chemokine CX3CL1
Monocytes
Transendothelial and Transepithelial Migration
Chemokine CCL2
Chemokine Receptors
Vascular System Injuries
Virus Diseases
Dendritic Cells

Keywords

  • Cell adhesion
  • Chemokine receptors
  • Chemokines
  • Chemotaxis
  • Inflammation

ASJC Scopus subject areas

  • Immunology

Cite this

Ancuta, P., Rao, R., Moses, A., Mehle, A., Shaw, S. K., Luscinskas, F. W., & Gabuzda, D. (2003). Fractalkine preferentially mediates arrest and migration of CD16+ monocytes. Journal of Experimental Medicine, 197(12), 1701-1707. https://doi.org/10.1084/jem.20022156

Fractalkine preferentially mediates arrest and migration of CD16+ monocytes. / Ancuta, Petronela; Rao, Ravi; Moses, Ashlee; Mehle, Andrew; Shaw, Sunil K.; Luscinskas, F. William; Gabuzda, Dana.

In: Journal of Experimental Medicine, Vol. 197, No. 12, 16.06.2003, p. 1701-1707.

Research output: Contribution to journalArticle

Ancuta, P, Rao, R, Moses, A, Mehle, A, Shaw, SK, Luscinskas, FW & Gabuzda, D 2003, 'Fractalkine preferentially mediates arrest and migration of CD16+ monocytes', Journal of Experimental Medicine, vol. 197, no. 12, pp. 1701-1707. https://doi.org/10.1084/jem.20022156
Ancuta, Petronela ; Rao, Ravi ; Moses, Ashlee ; Mehle, Andrew ; Shaw, Sunil K. ; Luscinskas, F. William ; Gabuzda, Dana. / Fractalkine preferentially mediates arrest and migration of CD16+ monocytes. In: Journal of Experimental Medicine. 2003 ; Vol. 197, No. 12. pp. 1701-1707.
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