Forskolin mimics the effects of calcitonin but not parathyroid hormone on bone resorption in vitro

R. F. Klein, R. A. Nissenson, G. J. Strewler

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Parathyroid hormone (PTH) and calcitonin stimulate bone adenylate cyclase activity and increase bone cAMP content, but PTH enhances and calcitonin inhibits bone resorption. This study examined the effects of forskolin, a non-hormonal activator of adenylate cyclase, on bone resorption and cAMP accumulation in 19-day fetal rat limb bones. Forskolin (10-9 to 10-5 M) stimulated bone cAMP generation in a concentration-dependent manner. However, in contrast to bPTH(1-34), which also stimulated cAMP accumulation, forskolin did not stimulate bone resorption. Moreover, forskolin did not augment the bone-resorbing activity of PTH even though it potentiated PTH stimulation of bone cAMP levels. Rather, high doses of forskolin (10-6 to 10-5 M) exhibited a calcitonin-like effect to inhibit PTH-mediated bone resorption. These results support a second-messenger function of cAMP for the inhibitory effects of calcitonin, but not for the stimulatory effects of PTH on bone resorption.

Original languageEnglish (US)
Pages (from-to)247-256
Number of pages10
JournalBone and Mineral
Issue number3
StatePublished - Jan 1 1988


ASJC Scopus subject areas

  • Surgery
  • Biochemistry
  • Endocrinology

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