Formation of two-dimensional crystals of histidine-tagged proteins using Ni2+-chelating lipid monolayers

Y. Rui, J. McDermott, E. Barklis, S. Wilkens, D. H. Thompson

Research output: Contribution to journalConference articlepeer-review


A system is developed for immobilizing histidine-tagged proteins onto lipid monolayers consisting of egg L-alpha-phosphatidylcholine (FPC) plus the novel synthetic lipid 1,2-di-O-hexadecyl-sn-glycero-3-N- (5-amino-1-carboxypenyl)iminodiacetic acid absorption spectrometry. Monolayer-bound, two-dimensional arrays of several N-terminal his tagged-proteins are examined by electron diffraction methods. The power and simplicity of the technique for determination of 2D protein structures are summarized.

Original languageEnglish (US)
Number of pages1
JournalPolymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering
StatePublished - Jan 1 1997
Externally publishedYes
EventProceedings of the 1997 Spring ACS Meeting - San Francisco, CA, USA
Duration: Apr 13 1997Apr 17 1997

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Polymers and Plastics


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