Formation of two-dimensional crystals of histidine-tagged proteins using Ni2+-chelating lipid monolayers

Y. Rui; J. McDermott; E. Barklis; S. Wilkens; D. H. Thompson

Formation of two-dimensional crystals of histidine-tagged proteins using Ni²⁺-chelating lipid monolayers

Y. Rui, J. McDermott, E. Barklis, S. Wilkens, D. H. Thompson

Research output: Contribution to journal › Conference article › peer-review

Abstract

A system is developed for immobilizing histidine-tagged proteins onto lipid monolayers consisting of egg L-alpha-phosphatidylcholine (FPC) plus the novel synthetic lipid 1,2-di-O-hexadecyl-sn-glycero-3-N- (5-amino-1-carboxypenyl)iminodiacetic acid absorption spectrometry. Monolayer-bound, two-dimensional arrays of several N-terminal his tagged-proteins are examined by electron diffraction methods. The power and simplicity of the technique for determination of 2D protein structures are summarized.

Original language	English (US)
Pages (from-to)	604
Number of pages	1
Journal	Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering
Volume	76
State	Published - 1997
Externally published	Yes
Event	Proceedings of the 1997 Spring ACS Meeting - San Francisco, CA, USA Duration: Apr 13 1997 → Apr 17 1997

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Polymers and Plastics

Cite this

Formation of two-dimensional crystals of histidine-tagged proteins using Ni²⁺-chelating lipid monolayers. / Rui, Y.; McDermott, J.; Barklis, E. et al.
In: Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering, Vol. 76, 1997, p. 604.

Research output: Contribution to journal › Conference article › peer-review

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T1 - Formation of two-dimensional crystals of histidine-tagged proteins using Ni2+-chelating lipid monolayers

AU - Rui, Y.

AU - McDermott, J.

AU - Barklis, E.

AU - Wilkens, S.

AU - Thompson, D. H.

PY - 1997

Y1 - 1997

N2 - A system is developed for immobilizing histidine-tagged proteins onto lipid monolayers consisting of egg L-alpha-phosphatidylcholine (FPC) plus the novel synthetic lipid 1,2-di-O-hexadecyl-sn-glycero-3-N- (5-amino-1-carboxypenyl)iminodiacetic acid absorption spectrometry. Monolayer-bound, two-dimensional arrays of several N-terminal his tagged-proteins are examined by electron diffraction methods. The power and simplicity of the technique for determination of 2D protein structures are summarized.

AB - A system is developed for immobilizing histidine-tagged proteins onto lipid monolayers consisting of egg L-alpha-phosphatidylcholine (FPC) plus the novel synthetic lipid 1,2-di-O-hexadecyl-sn-glycero-3-N- (5-amino-1-carboxypenyl)iminodiacetic acid absorption spectrometry. Monolayer-bound, two-dimensional arrays of several N-terminal his tagged-proteins are examined by electron diffraction methods. The power and simplicity of the technique for determination of 2D protein structures are summarized.

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M3 - Conference article

AN - SCOPUS:0030642720

SN - 0743-0515

VL - 76

SP - 604

JO - Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering

JF - Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering

T2 - Proceedings of the 1997 Spring ACS Meeting

Y2 - 13 April 1997 through 17 April 1997

ER -

Formation of two-dimensional crystals of histidine-tagged proteins using Ni²⁺-chelating lipid monolayers

Abstract

ASJC Scopus subject areas

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