Formation of two-dimensional crystals of histidine-tagged proteins using Ni2+-chelating lipid monolayers

Y. Rui, J. McDermott, Eric Barklis, S. Wilkens, D. H. Thompson

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A system is developed for immobilizing histidine-tagged proteins onto lipid monolayers consisting of egg L-alpha-phosphatidylcholine (FPC) plus the novel synthetic lipid 1,2-di-O-hexadecyl-sn-glycero-3-N- (5-amino-1-carboxypenyl)iminodiacetic acid absorption spectrometry. Monolayer-bound, two-dimensional arrays of several N-terminal his tagged-proteins are examined by electron diffraction methods. The power and simplicity of the technique for determination of 2D protein structures are summarized.

Original languageEnglish (US)
Title of host publicationPolymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering
Editors Anon
PublisherACS
Pages604
Number of pages1
Volume76
StatePublished - 1997
Externally publishedYes
EventProceedings of the 1997 Spring ACS Meeting - San Francisco, CA, USA
Duration: Apr 13 1997Apr 17 1997

Other

OtherProceedings of the 1997 Spring ACS Meeting
CitySan Francisco, CA, USA
Period4/13/974/17/97

Fingerprint

Chelation
Histidine
Lipids
Monolayers
Proteins
Crystals
Phosphatidylcholines
Electron diffraction
Spectrometry
Acids

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Polymers and Plastics

Cite this

Rui, Y., McDermott, J., Barklis, E., Wilkens, S., & Thompson, D. H. (1997). Formation of two-dimensional crystals of histidine-tagged proteins using Ni2+-chelating lipid monolayers. In Anon (Ed.), Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering (Vol. 76, pp. 604). ACS.

Formation of two-dimensional crystals of histidine-tagged proteins using Ni2+-chelating lipid monolayers. / Rui, Y.; McDermott, J.; Barklis, Eric; Wilkens, S.; Thompson, D. H.

Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering. ed. / Anon. Vol. 76 ACS, 1997. p. 604.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Rui, Y, McDermott, J, Barklis, E, Wilkens, S & Thompson, DH 1997, Formation of two-dimensional crystals of histidine-tagged proteins using Ni2+-chelating lipid monolayers. in Anon (ed.), Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering. vol. 76, ACS, pp. 604, Proceedings of the 1997 Spring ACS Meeting, San Francisco, CA, USA, 4/13/97.
Rui Y, McDermott J, Barklis E, Wilkens S, Thompson DH. Formation of two-dimensional crystals of histidine-tagged proteins using Ni2+-chelating lipid monolayers. In Anon, editor, Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering. Vol. 76. ACS. 1997. p. 604
Rui, Y. ; McDermott, J. ; Barklis, Eric ; Wilkens, S. ; Thompson, D. H. / Formation of two-dimensional crystals of histidine-tagged proteins using Ni2+-chelating lipid monolayers. Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering. editor / Anon. Vol. 76 ACS, 1997. pp. 604
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