Formation of 8-hydroxydeoxyguanosine within DNA of mouse keratinocytes exposed in culture to UVB and H2O2

Blake C. Beehler, Joseph Przybyszewski, Harold B. Box, Molly Kulesz-Martin

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

Exposure to UV light contributes to the development of skin cancer. The importance of reactive oxygen species in UV-radiation carcinogenesis has been recognized for some time and several associated DNA base modifications have been identified. In particular, 8-hydroxydeoxyguanosine (8-OHdG) has been well studied as an indicator of oxidative damage to calf thymus DNA exposed to a variety of oxygen-generating systems, including UV light. However, to date, few studies of 8-OHdG have been conducted in cell or animal systems and those in vitro investigations that studied UV exposure have used UVC (2 (dose-response range: 210-630 mJ/cm2). UVB exposure at 630 mJ/cm2 combined with 5 mM H2O2 elevated 8-OHdG formation up to 280% above that in control cells, whereas H2O2 alone had no effect. These results suggest that factors which increase the generation of reactive oxygen species by UV light may be potent cofactors of UV-radiation carcinogenesis.

Original languageEnglish (US)
Pages (from-to)2003-2007
Number of pages5
JournalCarcinogenesis
Volume13
Issue number11
StatePublished - Nov 1992
Externally publishedYes

Fingerprint

Ultraviolet Rays
Keratinocytes
Ultraviolet radiation
Reactive Oxygen Species
Mouse
DNA
Carcinogenesis
Radiation
Cofactor
Thymus
Dose-response
Cell
Oxygen
Skin Neoplasms
Skin
Animals
Cancer
Damage
Range of data
Culture

ASJC Scopus subject areas

  • Cancer Research
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Physiology
  • Behavioral Neuroscience

Cite this

Formation of 8-hydroxydeoxyguanosine within DNA of mouse keratinocytes exposed in culture to UVB and H2O2 . / Beehler, Blake C.; Przybyszewski, Joseph; Box, Harold B.; Kulesz-Martin, Molly.

In: Carcinogenesis, Vol. 13, No. 11, 11.1992, p. 2003-2007.

Research output: Contribution to journalArticle

Beehler, Blake C. ; Przybyszewski, Joseph ; Box, Harold B. ; Kulesz-Martin, Molly. / Formation of 8-hydroxydeoxyguanosine within DNA of mouse keratinocytes exposed in culture to UVB and H2O2 . In: Carcinogenesis. 1992 ; Vol. 13, No. 11. pp. 2003-2007.
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