Formation and degradation of deamido-TRH (pyroglutamyl-histidyl-proline) in rat brain after intraventricular injection of TRH

Eltot R. Spindel, Michael Lakher, Wurtman Richard J.

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Thyrotropin-releasing hormone (TRH) injected into the lateral ventricle of the rat's brain was rapidly metabolized to deamido-TRH (DA-TRH). Brain levels of TRH decreased with a half-life of 7 min, while the DA-TRH formed from it disappeared with a half-life of 2.5 min. To prevent the post-mortem degradation of DA-TRH it was necessary to sacrifice rats by directing focused microwave irradiation towards the brain. The short half-lives determined for TRH and DA-TRH in vivo were much shorter than those obtained using in vitro techniques. The in vivo formation and accumulation of DA-TRH was inhibited by bacitracin and unaffected by probenicid.

Original languageEnglish (US)
Pages (from-to)343-350
Number of pages8
JournalBrain research
Volume216
Issue number2
DOIs
StatePublished - Jul 20 1981
Externally publishedYes

Keywords

  • TRH
  • TRH metabolism
  • TRH turnover
  • deamido-TRH
  • neuropeptide metabolism

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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