Formation and degradation of deamido-TRH (pyroglutamyl-histidyl-proline) in rat brain after intraventricular injection of TRH

Eltot R. Spindel, Michael Lakher, Wurtman Richard J.

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    Abstract

    Thyrotropin-releasing hormone (TRH) injected into the lateral ventricle of the rat's brain was rapidly metabolized to deamido-TRH (DA-TRH). Brain levels of TRH decreased with a half-life of 7 min, while the DA-TRH formed from it disappeared with a half-life of 2.5 min. To prevent the post-mortem degradation of DA-TRH it was necessary to sacrifice rats by directing focused microwave irradiation towards the brain. The short half-lives determined for TRH and DA-TRH in vivo were much shorter than those obtained using in vitro techniques. The in vivo formation and accumulation of DA-TRH was inhibited by bacitracin and unaffected by probenicid.

    Original languageEnglish (US)
    Pages (from-to)343-350
    Number of pages8
    JournalBrain research
    Volume216
    Issue number2
    DOIs
    StatePublished - Jul 20 1981

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    Keywords

    • TRH
    • TRH metabolism
    • TRH turnover
    • deamido-TRH
    • neuropeptide metabolism

    ASJC Scopus subject areas

    • Neuroscience(all)
    • Molecular Biology
    • Clinical Neurology
    • Developmental Biology

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