Formation and degradation of deamido-TRH (pyroglutamyl-histidyl-proline) in rat brain after intraventricular injection of TRH

Eliot Spindel, Michael Lakher, Wurtman Richard J.

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Thyrotropin-releasing hormone (TRH) injected into the lateral ventricle of the rat's brain was rapidly metabolized to deamido-TRH (DA-TRH). Brain levels of TRH decreased with a half-life of 7 min, while the DA-TRH formed from it disappeared with a half-life of 2.5 min. To prevent the post-mortem degradation of DA-TRH it was necessary to sacrifice rats by directing focused microwave irradiation towards the brain. The short half-lives determined for TRH and DA-TRH in vivo were much shorter than those obtained using in vitro techniques. The in vivo formation and accumulation of DA-TRH was inhibited by bacitracin and unaffected by probenicid.

Original languageEnglish (US)
Pages (from-to)343-350
Number of pages8
JournalBrain Research
Volume216
Issue number2
DOIs
StatePublished - Jul 20 1981
Externally publishedYes

Fingerprint

Intraventricular Injections
Thyrotropin-Releasing Hormone
Brain
Half-Life
Bacitracin
Lateral Ventricles
Microwaves
deamido-thyrotropin-releasing hormone

Keywords

  • deamido-TRH
  • neuropeptide metabolism
  • TRH
  • TRH metabolism
  • TRH turnover

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Formation and degradation of deamido-TRH (pyroglutamyl-histidyl-proline) in rat brain after intraventricular injection of TRH. / Spindel, Eliot; Lakher, Michael; Richard J., Wurtman.

In: Brain Research, Vol. 216, No. 2, 20.07.1981, p. 343-350.

Research output: Contribution to journalArticle

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