TY - JOUR
T1 - Follicular lymphomas with and without translocation t(14;18) differ in gene expression profiles and genetic alterations
AU - Leich, Ellen
AU - Salaverria, Itziar
AU - Bea, Silvia
AU - Zettl, Andreas
AU - Wright, George
AU - Moreno, Victor
AU - Gascoyne, Randy D.
AU - Chan, Wing Chung
AU - Braziel, Rita M.
AU - Rimsza, Lisa M.
AU - Weisenburger, Dennis D.
AU - Delabie, Jan
AU - Jaffe, Elaine S.
AU - Lister, Andrew
AU - Fitzgibbon, Jude
AU - Staudt, Louis M.
AU - Hartmann, Elena M.
AU - Mueller-Hermelink, Hans Konrad
AU - Campo, Elias
AU - Ott, German
AU - Rosenwald, Andreas
PY - 2009
Y1 - 2009
N2 - Follicular lymphoma (FL) is genetically characterized by the presence of the t(14;18)(q32; q21) chromosomal translocation in approximately 90% of cases. In contrast to FL carrying the t(14;18), their t(14;18)-negative counterparts are less well studied about their immunohistochemical, genetic, molecular, and clinical features. Within a previously published series of 184 FLs grades 1 to 3A with available gene expression data, we identified 17 FLs lacking the t(14;18). Comparative genomic hybridization and high-resolution single nucleotide polymorphism (SNP) array profiling showed that gains/amplifications of the BCL2 gene locus in 18q were restricted to the t(14;18)-positive FL subgroup. Acomparison of gene expression profiles showed an enrichment of germinal center B cell-associated signatures in t(14;18)-positive FL, whereas activated B cell-like, NFκB, proliferation, and bystander cell signatures were enriched in t(14;18)-negative FL. These findings were confirmed by immunohistochemistry in an independent validation series of 84 FLs, in which 32% of t(14;18)-negative FLs showed weak or absent CD10 expression and 91% an increased Ki67 proliferation rate. Although overall survival did not differ between FL with and without t(14;18), our findings suggest distinct molecular features of t(14;18)-negative FL.
AB - Follicular lymphoma (FL) is genetically characterized by the presence of the t(14;18)(q32; q21) chromosomal translocation in approximately 90% of cases. In contrast to FL carrying the t(14;18), their t(14;18)-negative counterparts are less well studied about their immunohistochemical, genetic, molecular, and clinical features. Within a previously published series of 184 FLs grades 1 to 3A with available gene expression data, we identified 17 FLs lacking the t(14;18). Comparative genomic hybridization and high-resolution single nucleotide polymorphism (SNP) array profiling showed that gains/amplifications of the BCL2 gene locus in 18q were restricted to the t(14;18)-positive FL subgroup. Acomparison of gene expression profiles showed an enrichment of germinal center B cell-associated signatures in t(14;18)-positive FL, whereas activated B cell-like, NFκB, proliferation, and bystander cell signatures were enriched in t(14;18)-negative FL. These findings were confirmed by immunohistochemistry in an independent validation series of 84 FLs, in which 32% of t(14;18)-negative FLs showed weak or absent CD10 expression and 91% an increased Ki67 proliferation rate. Although overall survival did not differ between FL with and without t(14;18), our findings suggest distinct molecular features of t(14;18)-negative FL.
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U2 - 10.1182/blood-2009-01-198580
DO - 10.1182/blood-2009-01-198580
M3 - Article
C2 - 19471018
AN - SCOPUS:68249137273
SN - 0006-4971
VL - 114
SP - 826
EP - 834
JO - Blood
JF - Blood
IS - 4
ER -