Abstract
Objective(s): To determine whether follicular fluid (FF) from women of advanced reproductive age had a relative deficiency of the angiogenic cytokine vascular endothelial growth factor/vascular permeability factor. Furthermore, we sought to determine whether luteinized granulosa cells secrete vascular endothelial growth factor/vascular permeability factor in response to hypoxia. Design: Retrospective cohort study. Setting: University teaching hospital. Patients: Women undergoing follicular aspiration after superovulation in preparation for IVF-ET. Women of advanced reproductive age consisted of 21 women ≤38 years old (range, 38 to 46 years); 15 subjects ≤30 years served as the control population. Intervention(s): Granulosa cells and FF were collected by transvaginal aspiration 35 hours after hCG. Granulosa cells from two women were cultured for 24 and 48 hours in M199 + 10% fetal bovine serum in 1% O2-5% CO2-94% N2 (hypoxic) or 95% air-5% CO2 (normoxic) without or with 0.1 mol/L cobalt chloride. Main Outcome Measure(s): Pooled FF vascular endothelial growth factor/vascular permeability factor concentrations and media vascular endothelial growth factor/vascular permeability factor accumulation at 24 and 48 hours were determined. Result(s): Follicular fluid vascular endothelial growth factor/vascular permeability factor concentrations were higher in advanced reproductive age women compared with younger women (3,735 ± 2,155 versus 2,205 ± 952 pg/mL, mean ± SD). Accumulation of vascular endothelial growth factor/vascular permeability factor at 24 and 48 hours was 391 ± 54 and 744 ± 2 pg/mL in media maintained in 5% CO2 and air. Cobalt chloride induced a marked increase in vascular endothelial growth factor/vascular permeability factor (2,008 ± 52 pg/mL at 24 hours and 3,630 ± 519 pg/mL at 48 hours). An intermediate but significant increase in vascular endothelial growth factor/vascular permeability factor (733 ± 35 pg/mL at 24 hours and 2,675 ± 864 pg/mL at 48 hours) was observed with 1% O2 compared with normoxic controls. Conclusion(s): After hMG and hCG administration the FF from women of advanced reproductive age showed increased vascular endothelial growth factor/vascular permeability factor concentrations compared with younger women. Increased vascular endothelial growth factor/vascular permeability factor concentrations could be consistent with a hypoxic environment within follicles of older women.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 607-612 |
| Number of pages | 6 |
| Journal | Fertility and Sterility |
| Volume | 68 |
| Issue number | 4 |
| DOIs | |
| State | Published - Oct 1997 |
| Externally published | Yes |
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Keywords
- Aging
- Follicular fluid
- Hypoxia
- Vascular endothelial growth factor/vascular permeability factor
ASJC Scopus subject areas
- Obstetrics and Gynecology
Cite this
Follicular fluid vascular endothelial growth factor concentrations are elevated in women of advanced reproductive age undergoing ovulation induction. / Friedman, Chad I.; Arbogast, Laura; Danforth, Douglas R.; Alak, Baha M.; Herbosa-Encarnacion, Cristina; Seifer, David.
In: Fertility and Sterility, Vol. 68, No. 4, 10.1997, p. 607-612.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Follicular fluid vascular endothelial growth factor concentrations are elevated in women of advanced reproductive age undergoing ovulation induction
AU - Friedman, Chad I.
AU - Arbogast, Laura
AU - Danforth, Douglas R.
AU - Alak, Baha M.
AU - Herbosa-Encarnacion, Cristina
AU - Seifer, David
PY - 1997/10
Y1 - 1997/10
N2 - Objective(s): To determine whether follicular fluid (FF) from women of advanced reproductive age had a relative deficiency of the angiogenic cytokine vascular endothelial growth factor/vascular permeability factor. Furthermore, we sought to determine whether luteinized granulosa cells secrete vascular endothelial growth factor/vascular permeability factor in response to hypoxia. Design: Retrospective cohort study. Setting: University teaching hospital. Patients: Women undergoing follicular aspiration after superovulation in preparation for IVF-ET. Women of advanced reproductive age consisted of 21 women ≤38 years old (range, 38 to 46 years); 15 subjects ≤30 years served as the control population. Intervention(s): Granulosa cells and FF were collected by transvaginal aspiration 35 hours after hCG. Granulosa cells from two women were cultured for 24 and 48 hours in M199 + 10% fetal bovine serum in 1% O2-5% CO2-94% N2 (hypoxic) or 95% air-5% CO2 (normoxic) without or with 0.1 mol/L cobalt chloride. Main Outcome Measure(s): Pooled FF vascular endothelial growth factor/vascular permeability factor concentrations and media vascular endothelial growth factor/vascular permeability factor accumulation at 24 and 48 hours were determined. Result(s): Follicular fluid vascular endothelial growth factor/vascular permeability factor concentrations were higher in advanced reproductive age women compared with younger women (3,735 ± 2,155 versus 2,205 ± 952 pg/mL, mean ± SD). Accumulation of vascular endothelial growth factor/vascular permeability factor at 24 and 48 hours was 391 ± 54 and 744 ± 2 pg/mL in media maintained in 5% CO2 and air. Cobalt chloride induced a marked increase in vascular endothelial growth factor/vascular permeability factor (2,008 ± 52 pg/mL at 24 hours and 3,630 ± 519 pg/mL at 48 hours). An intermediate but significant increase in vascular endothelial growth factor/vascular permeability factor (733 ± 35 pg/mL at 24 hours and 2,675 ± 864 pg/mL at 48 hours) was observed with 1% O2 compared with normoxic controls. Conclusion(s): After hMG and hCG administration the FF from women of advanced reproductive age showed increased vascular endothelial growth factor/vascular permeability factor concentrations compared with younger women. Increased vascular endothelial growth factor/vascular permeability factor concentrations could be consistent with a hypoxic environment within follicles of older women.
AB - Objective(s): To determine whether follicular fluid (FF) from women of advanced reproductive age had a relative deficiency of the angiogenic cytokine vascular endothelial growth factor/vascular permeability factor. Furthermore, we sought to determine whether luteinized granulosa cells secrete vascular endothelial growth factor/vascular permeability factor in response to hypoxia. Design: Retrospective cohort study. Setting: University teaching hospital. Patients: Women undergoing follicular aspiration after superovulation in preparation for IVF-ET. Women of advanced reproductive age consisted of 21 women ≤38 years old (range, 38 to 46 years); 15 subjects ≤30 years served as the control population. Intervention(s): Granulosa cells and FF were collected by transvaginal aspiration 35 hours after hCG. Granulosa cells from two women were cultured for 24 and 48 hours in M199 + 10% fetal bovine serum in 1% O2-5% CO2-94% N2 (hypoxic) or 95% air-5% CO2 (normoxic) without or with 0.1 mol/L cobalt chloride. Main Outcome Measure(s): Pooled FF vascular endothelial growth factor/vascular permeability factor concentrations and media vascular endothelial growth factor/vascular permeability factor accumulation at 24 and 48 hours were determined. Result(s): Follicular fluid vascular endothelial growth factor/vascular permeability factor concentrations were higher in advanced reproductive age women compared with younger women (3,735 ± 2,155 versus 2,205 ± 952 pg/mL, mean ± SD). Accumulation of vascular endothelial growth factor/vascular permeability factor at 24 and 48 hours was 391 ± 54 and 744 ± 2 pg/mL in media maintained in 5% CO2 and air. Cobalt chloride induced a marked increase in vascular endothelial growth factor/vascular permeability factor (2,008 ± 52 pg/mL at 24 hours and 3,630 ± 519 pg/mL at 48 hours). An intermediate but significant increase in vascular endothelial growth factor/vascular permeability factor (733 ± 35 pg/mL at 24 hours and 2,675 ± 864 pg/mL at 48 hours) was observed with 1% O2 compared with normoxic controls. Conclusion(s): After hMG and hCG administration the FF from women of advanced reproductive age showed increased vascular endothelial growth factor/vascular permeability factor concentrations compared with younger women. Increased vascular endothelial growth factor/vascular permeability factor concentrations could be consistent with a hypoxic environment within follicles of older women.
KW - Aging
KW - Follicular fluid
KW - Hypoxia
KW - Vascular endothelial growth factor/vascular permeability factor
UR - http://www.scopus.com/inward/record.url?scp=0343053660&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0343053660&partnerID=8YFLogxK
U2 - 10.1016/S0015-0282(97)00278-1
DO - 10.1016/S0015-0282(97)00278-1
M3 - Article
VL - 68
SP - 607
EP - 612
JO - Fertility and Sterility
JF - Fertility and Sterility
SN - 0015-0282
IS - 4
ER -