Follicular fluid vascular endothelial growth factor concentrations are elevated in women of advanced reproductive age undergoing ovulation induction

Chad I. Friedman, Laura Arbogast, Douglas R. Danforth, Baha M. Alak, Cristina Herbosa-Encarnacion, David Seifer

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

Objective(s): To determine whether follicular fluid (FF) from women of advanced reproductive age had a relative deficiency of the angiogenic cytokine vascular endothelial growth factor/vascular permeability factor. Furthermore, we sought to determine whether luteinized granulosa cells secrete vascular endothelial growth factor/vascular permeability factor in response to hypoxia. Design: Retrospective cohort study. Setting: University teaching hospital. Patients: Women undergoing follicular aspiration after superovulation in preparation for IVF-ET. Women of advanced reproductive age consisted of 21 women ≤38 years old (range, 38 to 46 years); 15 subjects ≤30 years served as the control population. Intervention(s): Granulosa cells and FF were collected by transvaginal aspiration 35 hours after hCG. Granulosa cells from two women were cultured for 24 and 48 hours in M199 + 10% fetal bovine serum in 1% O2-5% CO2-94% N2 (hypoxic) or 95% air-5% CO2 (normoxic) without or with 0.1 mol/L cobalt chloride. Main Outcome Measure(s): Pooled FF vascular endothelial growth factor/vascular permeability factor concentrations and media vascular endothelial growth factor/vascular permeability factor accumulation at 24 and 48 hours were determined. Result(s): Follicular fluid vascular endothelial growth factor/vascular permeability factor concentrations were higher in advanced reproductive age women compared with younger women (3,735 ± 2,155 versus 2,205 ± 952 pg/mL, mean ± SD). Accumulation of vascular endothelial growth factor/vascular permeability factor at 24 and 48 hours was 391 ± 54 and 744 ± 2 pg/mL in media maintained in 5% CO2 and air. Cobalt chloride induced a marked increase in vascular endothelial growth factor/vascular permeability factor (2,008 ± 52 pg/mL at 24 hours and 3,630 ± 519 pg/mL at 48 hours). An intermediate but significant increase in vascular endothelial growth factor/vascular permeability factor (733 ± 35 pg/mL at 24 hours and 2,675 ± 864 pg/mL at 48 hours) was observed with 1% O2 compared with normoxic controls. Conclusion(s): After hMG and hCG administration the FF from women of advanced reproductive age showed increased vascular endothelial growth factor/vascular permeability factor concentrations compared with younger women. Increased vascular endothelial growth factor/vascular permeability factor concentrations could be consistent with a hypoxic environment within follicles of older women.

Original languageEnglish (US)
Pages (from-to)607-612
Number of pages6
JournalFertility and Sterility
Volume68
Issue number4
DOIs
StatePublished - Oct 1997
Externally publishedYes

Fingerprint

Follicular Fluid
Ovulation Induction
Vascular Endothelial Growth Factor A
Granulosa Cells
Air
Superovulation

Keywords

  • Aging
  • Follicular fluid
  • Hypoxia
  • Vascular endothelial growth factor/vascular permeability factor

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Follicular fluid vascular endothelial growth factor concentrations are elevated in women of advanced reproductive age undergoing ovulation induction. / Friedman, Chad I.; Arbogast, Laura; Danforth, Douglas R.; Alak, Baha M.; Herbosa-Encarnacion, Cristina; Seifer, David.

In: Fertility and Sterility, Vol. 68, No. 4, 10.1997, p. 607-612.

Research output: Contribution to journalArticle

Friedman, Chad I. ; Arbogast, Laura ; Danforth, Douglas R. ; Alak, Baha M. ; Herbosa-Encarnacion, Cristina ; Seifer, David. / Follicular fluid vascular endothelial growth factor concentrations are elevated in women of advanced reproductive age undergoing ovulation induction. In: Fertility and Sterility. 1997 ; Vol. 68, No. 4. pp. 607-612.
@article{c3e16ebb903d4995a6be578277bde279,
title = "Follicular fluid vascular endothelial growth factor concentrations are elevated in women of advanced reproductive age undergoing ovulation induction",
abstract = "Objective(s): To determine whether follicular fluid (FF) from women of advanced reproductive age had a relative deficiency of the angiogenic cytokine vascular endothelial growth factor/vascular permeability factor. Furthermore, we sought to determine whether luteinized granulosa cells secrete vascular endothelial growth factor/vascular permeability factor in response to hypoxia. Design: Retrospective cohort study. Setting: University teaching hospital. Patients: Women undergoing follicular aspiration after superovulation in preparation for IVF-ET. Women of advanced reproductive age consisted of 21 women ≤38 years old (range, 38 to 46 years); 15 subjects ≤30 years served as the control population. Intervention(s): Granulosa cells and FF were collected by transvaginal aspiration 35 hours after hCG. Granulosa cells from two women were cultured for 24 and 48 hours in M199 + 10{\%} fetal bovine serum in 1{\%} O2-5{\%} CO2-94{\%} N2 (hypoxic) or 95{\%} air-5{\%} CO2 (normoxic) without or with 0.1 mol/L cobalt chloride. Main Outcome Measure(s): Pooled FF vascular endothelial growth factor/vascular permeability factor concentrations and media vascular endothelial growth factor/vascular permeability factor accumulation at 24 and 48 hours were determined. Result(s): Follicular fluid vascular endothelial growth factor/vascular permeability factor concentrations were higher in advanced reproductive age women compared with younger women (3,735 ± 2,155 versus 2,205 ± 952 pg/mL, mean ± SD). Accumulation of vascular endothelial growth factor/vascular permeability factor at 24 and 48 hours was 391 ± 54 and 744 ± 2 pg/mL in media maintained in 5{\%} CO2 and air. Cobalt chloride induced a marked increase in vascular endothelial growth factor/vascular permeability factor (2,008 ± 52 pg/mL at 24 hours and 3,630 ± 519 pg/mL at 48 hours). An intermediate but significant increase in vascular endothelial growth factor/vascular permeability factor (733 ± 35 pg/mL at 24 hours and 2,675 ± 864 pg/mL at 48 hours) was observed with 1{\%} O2 compared with normoxic controls. Conclusion(s): After hMG and hCG administration the FF from women of advanced reproductive age showed increased vascular endothelial growth factor/vascular permeability factor concentrations compared with younger women. Increased vascular endothelial growth factor/vascular permeability factor concentrations could be consistent with a hypoxic environment within follicles of older women.",
keywords = "Aging, Follicular fluid, Hypoxia, Vascular endothelial growth factor/vascular permeability factor",
author = "Friedman, {Chad I.} and Laura Arbogast and Danforth, {Douglas R.} and Alak, {Baha M.} and Cristina Herbosa-Encarnacion and David Seifer",
year = "1997",
month = "10",
doi = "10.1016/S0015-0282(97)00278-1",
language = "English (US)",
volume = "68",
pages = "607--612",
journal = "Fertility and Sterility",
issn = "0015-0282",
publisher = "Elsevier Inc.",
number = "4",

}

TY - JOUR

T1 - Follicular fluid vascular endothelial growth factor concentrations are elevated in women of advanced reproductive age undergoing ovulation induction

AU - Friedman, Chad I.

AU - Arbogast, Laura

AU - Danforth, Douglas R.

AU - Alak, Baha M.

AU - Herbosa-Encarnacion, Cristina

AU - Seifer, David

PY - 1997/10

Y1 - 1997/10

N2 - Objective(s): To determine whether follicular fluid (FF) from women of advanced reproductive age had a relative deficiency of the angiogenic cytokine vascular endothelial growth factor/vascular permeability factor. Furthermore, we sought to determine whether luteinized granulosa cells secrete vascular endothelial growth factor/vascular permeability factor in response to hypoxia. Design: Retrospective cohort study. Setting: University teaching hospital. Patients: Women undergoing follicular aspiration after superovulation in preparation for IVF-ET. Women of advanced reproductive age consisted of 21 women ≤38 years old (range, 38 to 46 years); 15 subjects ≤30 years served as the control population. Intervention(s): Granulosa cells and FF were collected by transvaginal aspiration 35 hours after hCG. Granulosa cells from two women were cultured for 24 and 48 hours in M199 + 10% fetal bovine serum in 1% O2-5% CO2-94% N2 (hypoxic) or 95% air-5% CO2 (normoxic) without or with 0.1 mol/L cobalt chloride. Main Outcome Measure(s): Pooled FF vascular endothelial growth factor/vascular permeability factor concentrations and media vascular endothelial growth factor/vascular permeability factor accumulation at 24 and 48 hours were determined. Result(s): Follicular fluid vascular endothelial growth factor/vascular permeability factor concentrations were higher in advanced reproductive age women compared with younger women (3,735 ± 2,155 versus 2,205 ± 952 pg/mL, mean ± SD). Accumulation of vascular endothelial growth factor/vascular permeability factor at 24 and 48 hours was 391 ± 54 and 744 ± 2 pg/mL in media maintained in 5% CO2 and air. Cobalt chloride induced a marked increase in vascular endothelial growth factor/vascular permeability factor (2,008 ± 52 pg/mL at 24 hours and 3,630 ± 519 pg/mL at 48 hours). An intermediate but significant increase in vascular endothelial growth factor/vascular permeability factor (733 ± 35 pg/mL at 24 hours and 2,675 ± 864 pg/mL at 48 hours) was observed with 1% O2 compared with normoxic controls. Conclusion(s): After hMG and hCG administration the FF from women of advanced reproductive age showed increased vascular endothelial growth factor/vascular permeability factor concentrations compared with younger women. Increased vascular endothelial growth factor/vascular permeability factor concentrations could be consistent with a hypoxic environment within follicles of older women.

AB - Objective(s): To determine whether follicular fluid (FF) from women of advanced reproductive age had a relative deficiency of the angiogenic cytokine vascular endothelial growth factor/vascular permeability factor. Furthermore, we sought to determine whether luteinized granulosa cells secrete vascular endothelial growth factor/vascular permeability factor in response to hypoxia. Design: Retrospective cohort study. Setting: University teaching hospital. Patients: Women undergoing follicular aspiration after superovulation in preparation for IVF-ET. Women of advanced reproductive age consisted of 21 women ≤38 years old (range, 38 to 46 years); 15 subjects ≤30 years served as the control population. Intervention(s): Granulosa cells and FF were collected by transvaginal aspiration 35 hours after hCG. Granulosa cells from two women were cultured for 24 and 48 hours in M199 + 10% fetal bovine serum in 1% O2-5% CO2-94% N2 (hypoxic) or 95% air-5% CO2 (normoxic) without or with 0.1 mol/L cobalt chloride. Main Outcome Measure(s): Pooled FF vascular endothelial growth factor/vascular permeability factor concentrations and media vascular endothelial growth factor/vascular permeability factor accumulation at 24 and 48 hours were determined. Result(s): Follicular fluid vascular endothelial growth factor/vascular permeability factor concentrations were higher in advanced reproductive age women compared with younger women (3,735 ± 2,155 versus 2,205 ± 952 pg/mL, mean ± SD). Accumulation of vascular endothelial growth factor/vascular permeability factor at 24 and 48 hours was 391 ± 54 and 744 ± 2 pg/mL in media maintained in 5% CO2 and air. Cobalt chloride induced a marked increase in vascular endothelial growth factor/vascular permeability factor (2,008 ± 52 pg/mL at 24 hours and 3,630 ± 519 pg/mL at 48 hours). An intermediate but significant increase in vascular endothelial growth factor/vascular permeability factor (733 ± 35 pg/mL at 24 hours and 2,675 ± 864 pg/mL at 48 hours) was observed with 1% O2 compared with normoxic controls. Conclusion(s): After hMG and hCG administration the FF from women of advanced reproductive age showed increased vascular endothelial growth factor/vascular permeability factor concentrations compared with younger women. Increased vascular endothelial growth factor/vascular permeability factor concentrations could be consistent with a hypoxic environment within follicles of older women.

KW - Aging

KW - Follicular fluid

KW - Hypoxia

KW - Vascular endothelial growth factor/vascular permeability factor

UR - http://www.scopus.com/inward/record.url?scp=0343053660&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0343053660&partnerID=8YFLogxK

U2 - 10.1016/S0015-0282(97)00278-1

DO - 10.1016/S0015-0282(97)00278-1

M3 - Article

VL - 68

SP - 607

EP - 612

JO - Fertility and Sterility

JF - Fertility and Sterility

SN - 0015-0282

IS - 4

ER -