Follicular development, ovulation, and corpus luteum formation in cryopreserved human ovarian tissue after xenotransplantation

S. Samuel Kim, Michael R. Soules, David Battaglia

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

Objective: To assess the competency of human frozen/thawed ovarian follicles matured in xenografts to form functioning corpora luteae after human chorionic gonadotropin (hCG) administration. Design: Prospective controlled animal study. Setting: University research laboratory. Patient(s): Three women (19, 28, and 36 years) who underwent oophorectomy. Animal(s): Nineteen female severe combined immunodeficient (SCID) mice. Intervention(s): Cryopreserved human ovarian tissues were grafted into the s.c. space of bilaterally oophorectomized SCID mice. All the animals were stimulated with pregnant mare's serum gonadotropin (PMSG) for 4 weeks starting from 16 weeks after transplantation. Twelve animals were injected with hCG at the end of gonadotropin stimulation. Main Outcome Measure(s): [1] The rate of grafts with growing follicles, with antral follicles, and/or with corpora luteae. [2] The histologic assessment of follicles and corpora luteae. [3] The serum progesterone and estradiol level in animals with corpus luteum in the grafts. Result(s): [1] The rate of grafts with growing follicles and with corpora luteae was 33% to 100%, and 28% to 50%, respectively. [2] Corpora luteae in xenografts were all morphologically normal. [3] The progesterone levels were all above 3.0 ng/mL. Conclusion(s): This study showed that the cryopreserved human ovarian follicles can be matured to a stage at which they can form functioning corpora luteae in the host animal.

Original languageEnglish (US)
Pages (from-to)77-82
Number of pages6
JournalFertility and Sterility
Volume78
Issue number1
DOIs
StatePublished - 2002

Fingerprint

Heterologous Transplantation
Corpus Luteum
Ovulation
Ovarian Follicle
SCID Mice
Chorionic Gonadotropin
Transplants
Heterografts
Progesterone
Equine Gonadotropins
Ovariectomy
Gonadotropins
Estradiol
Transplantation
Outcome Assessment (Health Care)
Serum
Research

Keywords

  • Corpus luteum
  • Cryopreservation
  • Follicular maturation
  • Ovarian tissue
  • SCID
  • Transplantation
  • Xenograft

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Follicular development, ovulation, and corpus luteum formation in cryopreserved human ovarian tissue after xenotransplantation. / Kim, S. Samuel; Soules, Michael R.; Battaglia, David.

In: Fertility and Sterility, Vol. 78, No. 1, 2002, p. 77-82.

Research output: Contribution to journalArticle

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N2 - Objective: To assess the competency of human frozen/thawed ovarian follicles matured in xenografts to form functioning corpora luteae after human chorionic gonadotropin (hCG) administration. Design: Prospective controlled animal study. Setting: University research laboratory. Patient(s): Three women (19, 28, and 36 years) who underwent oophorectomy. Animal(s): Nineteen female severe combined immunodeficient (SCID) mice. Intervention(s): Cryopreserved human ovarian tissues were grafted into the s.c. space of bilaterally oophorectomized SCID mice. All the animals were stimulated with pregnant mare's serum gonadotropin (PMSG) for 4 weeks starting from 16 weeks after transplantation. Twelve animals were injected with hCG at the end of gonadotropin stimulation. Main Outcome Measure(s): [1] The rate of grafts with growing follicles, with antral follicles, and/or with corpora luteae. [2] The histologic assessment of follicles and corpora luteae. [3] The serum progesterone and estradiol level in animals with corpus luteum in the grafts. Result(s): [1] The rate of grafts with growing follicles and with corpora luteae was 33% to 100%, and 28% to 50%, respectively. [2] Corpora luteae in xenografts were all morphologically normal. [3] The progesterone levels were all above 3.0 ng/mL. Conclusion(s): This study showed that the cryopreserved human ovarian follicles can be matured to a stage at which they can form functioning corpora luteae in the host animal.

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