Follicle-stimulating hormone regulation of A-kinase anchoring proteins in granulosa cells

D. W. Carr, D. A. DeManno, A. Atwood, M. Hunzicker-Dunn, J. D. Scott

Research output: Contribution to journalArticle

67 Scopus citations

Abstract

It has been well established that the biochemical and morphological changes during maturation of granulosa cells that are induced by follicle- stimulating hormone (FSH) occur through the elevation of intracellular cAMP and consequent activation of the cAMP-dependent protein kinase (PKA). In this report we show that FSH action alters the expression of A-Kinase Anchoring Proteins (AKAPs), which function to target the subcellular distribution of the type II PKA. Exposure of granulosa cells grown in primary culture with FSH and estradiol for 72 h resulted in the up-regulation of an 80-kDa AKAP and the RIIβ subunit of PKA, whereas cells grown in control medium containing only estradiol produced a time-dependent increase of a 140-kDa AKAP. RII overlays performed with [32P]RIIα preferentially detected RII- binding bands of 80 and 95 kDa compared to blots probed with [32P]RIIβ, suggesting that FSH may alter the subcellular location of PKA in an isoform- specific manner. FSH treatment causes a translocation of RIIα from the particulate to the cytosolic fraction coincident with the induction of the 80-kDa AKAP, which is also predominately cytosolic. These data suggest that FSH promotes a redistribution of the type II PKA holoenzyme through the selective induction of an RII isoform-specific AKAP.

Original languageEnglish (US)
Pages (from-to)20729-20732
Number of pages4
JournalJournal of Biological Chemistry
Volume268
Issue number28
StatePublished - 1993

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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