Folding pathway mediated by an intramolecular chaperone. The inhibitory and chaperone functions of the subtilisin propeptide are not obligatorily linked

Xuan Fu, Masayori Inouye, Ujwal Shinde

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

The subtilisin propeptide functions as an intramolecular chaperone (IMC) that facilitates correct folding of the catalytic domain while acting like a competitive inhibitor of proteolytic activity. Upon completion of folding, subtilisin initiates IMC degradation to complete precursor maturation. Existing data suggest that the chaperone and inhibitory functions of the subtilisin IMC domain are interdependent during folding. Based on x-ray structure of the IMC-subtilisin complex, we introduce a point mutation (E112A) to disrupt three hydrogen bonds that stabilize the interface between the protease and its IMC domain. This mutation within subtilisin does not alter the folding kinetics but dramatically slows down autoprocessing of the IMC domain. Inhibition of E112A-subtilisin activity by the IMC added in trans is 35-fold weaker than wild-type subtilisin. Although the IMC domain displays substantial loss of inhibitory function, its ability to chaperone E112A- subtilisin folding remains intact. Our results show that (i) the chaperone activity of the IMC domain is not obligatorily linked with its ability to bind with and inhibit active subtilisin; (ii) degradation and not autoprocessing of the IMC domain is the rate-limiting step in precursor maturation; and (iii) the Glu112 residue within the IMC-subtilisin interface is not crucial for initiating folding but is important in maintaining the IMC structure capable of binding subtilisin.

Original languageEnglish (US)
Pages (from-to)16871-16878
Number of pages8
JournalJournal of Biological Chemistry
Volume275
Issue number22
DOIs
StatePublished - Jun 2 2000
Externally publishedYes

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Subtilisin
Degradation
Point Mutation
Hydrogen
Catalytic Domain
Hydrogen bonds
Peptide Hydrolases
X-Rays

ASJC Scopus subject areas

  • Biochemistry

Cite this

Folding pathway mediated by an intramolecular chaperone. The inhibitory and chaperone functions of the subtilisin propeptide are not obligatorily linked. / Fu, Xuan; Inouye, Masayori; Shinde, Ujwal.

In: Journal of Biological Chemistry, Vol. 275, No. 22, 02.06.2000, p. 16871-16878.

Research output: Contribution to journalArticle

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