Folding mediated by an intramolecular chaperone: Autoprocessing pathway of the precursor resolved via a substrate assisted catalysis mechanism

Ujwal Shinde, Masayori Inouye

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

Subtilisin is synthesized with an N-terminal propeptide which has been demonstrated to function as an intramolecular chaperone that is only essential for the folding of the active enzyme. After folding, the propeptide is removed via an intramolecular autoprocessing mechanism. This mechanism is blocked when His64, a member of the catalytic triad is substituted with Ala. However, an additional mutation in the propeptide substituting Glu-2 with His was able to suppress the His64Ala mutation, allowing autoprocessing of the propeptide. This suppression is considered to be due to a 'substrate assisted catalysis' mechanism and demonstrates that the cleavage to the subtilisin propeptide is an autocatalytic process.

Original languageEnglish (US)
Pages (from-to)390-395
Number of pages6
JournalJournal of molecular biology
Volume247
Issue number3
DOIs
StatePublished - 1995
Externally publishedYes

Keywords

  • Autoprocessing mechanism
  • Intramolecular chaperone
  • Propeptide
  • Protein folding
  • Substrate assisted catalysis

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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