Focal expression of insulin-like growth factor I in rat kidney collecting duct

J. D. Bortz, P. Rotwein, D. DeVol, P. J. Bechtel, V. A. Hansen, M. R. Hammerman

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Abstract

To address the question of insulin-like growth factor (IGF) I localization and synthesis in kidney, we used two complementary experimental approaches: immunohistochemistry of fixed paraffin-embedded rat kidney sections; and measurement of IGF I mRNA in isolated components of the rat nephron, using a highly sensitive and specific solution hybridization assay. Immunostainable IGF I was localized exclusively to principal cells of cortical and medullary collecting ducts. Administration of growth hormone to hypophysectomized rats for 8 d resulted in enhanced immunohistochemical staining of IGF I within collecting ducts, but no detectable IGF I in other portions of the nephron. The abundance of IGF I mRNA was 7-12-fold higher in isolated papillary collecting ducts than in proximal tubules or glomeruli, and was enriched 10-fold compared with whole kidney. Our data demonstrate colocalization of IGF I and IGF I mRNA in the collecting duct, consistent with focal expression of the IGF I gene at this site.

Original languageEnglish (US)
Pages (from-to)811-819
Number of pages9
JournalJournal of Cell Biology
Volume107
Issue number2
DOIs
StatePublished - 1988

ASJC Scopus subject areas

  • Cell Biology

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    Bortz, J. D., Rotwein, P., DeVol, D., Bechtel, P. J., Hansen, V. A., & Hammerman, M. R. (1988). Focal expression of insulin-like growth factor I in rat kidney collecting duct. Journal of Cell Biology, 107(2), 811-819. https://doi.org/10.1083/jcb.107.2.811