Fluorescent nucleic add hybridization methods

D. Pinkel, Joe Gray, R. Segraves, F. Waldman, B. Trask, L. C. Yu, D. Eastmond, P. Dean

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In the last several years a number of approaches have been developed that permit detection of nucleic acid hybridization using fluorescence techniques. These have several advantages over the previously available radioactive procedures. Among these are much higher spatial resolution, the speed with which the results are available, the reduced hazard, and the possibility of distinguishing the binding of multiple probes using different fluorochromes. Exploitation of this potential requires application of optical techniques of increasing sophistication such as development of new fluorophores, improved methods of visualizing multiple dyes in the same sample, application of image processing to extract quantitative information, and optimized optical sectioning for three dimensional reconstructions.

Original languageEnglish (US)
Pages (from-to)123-132
Number of pages10
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume1063
DOIs
StatePublished - Jun 13 1989
Externally publishedYes

Fingerprint

Three-dimensional Reconstruction
Fluorophores
Nucleic acids
nucleic acids
exploitation
Dyes
Fluorescent Dyes
Spatial Resolution
Hazard
Fluorescence
Exploitation
Nucleic Acids
hazards
image processing
Image Processing
Hazards
Image processing
Probe
Coloring Agents
High Resolution

ASJC Scopus subject areas

  • Applied Mathematics
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering
  • Computer Science Applications

Cite this

Fluorescent nucleic add hybridization methods. / Pinkel, D.; Gray, Joe; Segraves, R.; Waldman, F.; Trask, B.; Yu, L. C.; Eastmond, D.; Dean, P.

In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 1063, 13.06.1989, p. 123-132.

Research output: Contribution to journalArticle

Pinkel, D, Gray, J, Segraves, R, Waldman, F, Trask, B, Yu, LC, Eastmond, D & Dean, P 1989, 'Fluorescent nucleic add hybridization methods', Proceedings of SPIE - The International Society for Optical Engineering, vol. 1063, pp. 123-132. https://doi.org/10.1117/12.951898
Pinkel, D. ; Gray, Joe ; Segraves, R. ; Waldman, F. ; Trask, B. ; Yu, L. C. ; Eastmond, D. ; Dean, P. / Fluorescent nucleic add hybridization methods. In: Proceedings of SPIE - The International Society for Optical Engineering. 1989 ; Vol. 1063. pp. 123-132.
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