Fluorescent imaging of cells and nucleic acids in bioelectronic chips

Jing Cheng, Lei Wu, Jonathan Diver, Paul Swanson, Adam Uribe, Michael Krihak, Jainamma Krotz, Kenneth A. Bloch, Bill Street, Trevor Young, Tony Llevares, Tim LeClair, Don Ackley, Michael (Mike) Heller, James P. O'Connell

Research output: Contribution to journalConference article

4 Citations (Scopus)

Abstract

We have performed separation of bacterial and cultured cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a `laboratory-on-a-chip' system will be presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.

Original languageEnglish (US)
Pages (from-to)23-28
Number of pages6
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume3600
StatePublished - Jan 1 1999
Externally publishedYes
EventProceedings of the 1999 Biomedical Imaging: Reporters, Dyes, and Instrumentation - San Jose, CA, USA
Duration: Jan 26 1999Jan 28 1999

Fingerprint

Nucleic acids
nucleic acids
Nucleic Acids
DNA
Chip
deoxyribonucleic acid
chips
Imaging
Dyes
Imaging techniques
acids
Cell
DNA Probes
Probe
dyes
Electrophoresis
cells
Fluorescent Dyes
chips (electronics)
Dielectrophoresis

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Cheng, J., Wu, L., Diver, J., Swanson, P., Uribe, A., Krihak, M., ... O'Connell, J. P. (1999). Fluorescent imaging of cells and nucleic acids in bioelectronic chips. Proceedings of SPIE - The International Society for Optical Engineering, 3600, 23-28.

Fluorescent imaging of cells and nucleic acids in bioelectronic chips. / Cheng, Jing; Wu, Lei; Diver, Jonathan; Swanson, Paul; Uribe, Adam; Krihak, Michael; Krotz, Jainamma; Bloch, Kenneth A.; Street, Bill; Young, Trevor; Llevares, Tony; LeClair, Tim; Ackley, Don; Heller, Michael (Mike); O'Connell, James P.

In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 3600, 01.01.1999, p. 23-28.

Research output: Contribution to journalConference article

Cheng, J, Wu, L, Diver, J, Swanson, P, Uribe, A, Krihak, M, Krotz, J, Bloch, KA, Street, B, Young, T, Llevares, T, LeClair, T, Ackley, D, Heller, MM & O'Connell, JP 1999, 'Fluorescent imaging of cells and nucleic acids in bioelectronic chips', Proceedings of SPIE - The International Society for Optical Engineering, vol. 3600, pp. 23-28.
Cheng, Jing ; Wu, Lei ; Diver, Jonathan ; Swanson, Paul ; Uribe, Adam ; Krihak, Michael ; Krotz, Jainamma ; Bloch, Kenneth A. ; Street, Bill ; Young, Trevor ; Llevares, Tony ; LeClair, Tim ; Ackley, Don ; Heller, Michael (Mike) ; O'Connell, James P. / Fluorescent imaging of cells and nucleic acids in bioelectronic chips. In: Proceedings of SPIE - The International Society for Optical Engineering. 1999 ; Vol. 3600. pp. 23-28.
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AU - Krotz, Jainamma

AU - Bloch, Kenneth A.

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AU - Young, Trevor

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