Fluorescent imaging of cells and nucleic acids in bioelectronic chips

Jing Cheng; Lei Wu; Jonathan Diver; Paul Swanson; Adam Uribe; Michael Krihak; Jainamma Krotz; Kenneth A. Bloch; Bill Street; Trevor Young; Tony Llevares; Tim LeClair; Don Ackley; Michael Heller; James P. O'Connell

Fluorescent imaging of cells and nucleic acids in bioelectronic chips

Jing Cheng, Lei Wu, Jonathan Diver, Paul Swanson, Adam Uribe, Michael Krihak, Jainamma Krotz, Kenneth A. Bloch, Bill Street, Trevor Young, Tony Llevares, Tim LeClair, Don Ackley, Michael Heller, James P. O'Connell

Research output: Contribution to journal › Conference article › peer-review

4 Scopus citations

Abstract

We have performed separation of bacterial and cultured cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a `laboratory-on-a-chip' system will be presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.

Original language	English (US)
Pages (from-to)	23-28
Number of pages	6
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	3600
State	Published - 1999
Externally published	Yes
Event	Proceedings of the 1999 Biomedical Imaging: Reporters, Dyes, and Instrumentation - San Jose, CA, USA Duration: Jan 26 1999 → Jan 28 1999

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

Cite this

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title = "Fluorescent imaging of cells and nucleic acids in bioelectronic chips",

abstract = "We have performed separation of bacterial and cultured cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a `laboratory-on-a-chip' system will be presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.",

author = "Jing Cheng and Lei Wu and Jonathan Diver and Paul Swanson and Adam Uribe and Michael Krihak and Jainamma Krotz and Bloch, {Kenneth A.} and Bill Street and Trevor Young and Tony Llevares and Tim LeClair and Don Ackley and Michael Heller and O'Connell, {James P.}",

year = "1999",

language = "English (US)",

volume = "3600",

pages = "23--28",

journal = "Proceedings of SPIE - The International Society for Optical Engineering",

issn = "0277-786X",

publisher = "SPIE",

note = "Proceedings of the 1999 Biomedical Imaging: Reporters, Dyes, and Instrumentation ; Conference date: 26-01-1999 Through 28-01-1999",

}

TY - JOUR

T1 - Fluorescent imaging of cells and nucleic acids in bioelectronic chips

AU - Cheng, Jing

AU - Wu, Lei

AU - Diver, Jonathan

AU - Swanson, Paul

AU - Uribe, Adam

AU - Krihak, Michael

AU - Krotz, Jainamma

AU - Bloch, Kenneth A.

AU - Street, Bill

AU - Young, Trevor

AU - Llevares, Tony

AU - LeClair, Tim

AU - Ackley, Don

AU - Heller, Michael

AU - O'Connell, James P.

PY - 1999

Y1 - 1999

N2 - We have performed separation of bacterial and cultured cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a `laboratory-on-a-chip' system will be presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.

AB - We have performed separation of bacterial and cultured cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a `laboratory-on-a-chip' system will be presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.

UR - http://www.scopus.com/inward/record.url?scp=0032642413&partnerID=8YFLogxK

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M3 - Conference article

AN - SCOPUS:0032642413

SN - 0277-786X

VL - 3600

SP - 23

EP - 28

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Proceedings of the 1999 Biomedical Imaging: Reporters, Dyes, and Instrumentation

Y2 - 26 January 1999 through 28 January 1999

ER -

Fluorescent imaging of cells and nucleic acids in bioelectronic chips

Abstract

ASJC Scopus subject areas

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