Fluorescence visualization of newly synthesized proteins in mammalian cells

Kimberly E. Beatty, Julie C. Liu, Fang Xie, Daniela C. Dieterich, Erin M. Schuman, Qian Wang, David A. Tirrell

Research output: Contribution to journalArticle

200 Scopus citations

Abstract

(Figure Presented) Playing tag: Noncanonical amino acid tagging enables the selective fluorescent visualization of newly synthesized proteins in mammalian cells (see the picture). Susceptibility to tagging is determined by the spatial and temporal character of the protein synthesis, thus providing a complement to methods which identify relevant members of the proteome.

Original languageEnglish (US)
Pages (from-to)7364-7367
Number of pages4
JournalAngewandte Chemie - International Edition
Volume45
Issue number44
DOIs
StatePublished - Nov 13 2006

Keywords

  • Alkynes
  • Azides
  • Fluorescent probes
  • Protein modifications
  • Proteomics

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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  • Cite this

    Beatty, K. E., Liu, J. C., Xie, F., Dieterich, D. C., Schuman, E. M., Wang, Q., & Tirrell, D. A. (2006). Fluorescence visualization of newly synthesized proteins in mammalian cells. Angewandte Chemie - International Edition, 45(44), 7364-7367. https://doi.org/10.1002/anie.200602114