Fluorescence spectroscopy of tissue: Recovery of intrinsic fluorescence from measured fluorescence

Craig M. Gardner, Steven L. Jacques, Ashley J. Welch

Research output: Contribution to journalArticle

118 Scopus citations

Abstract

We present a method for recovering the intrinsic fluorescence coefficient, defined as the product of the fluorophore absorption coefficient and the fluorescence energy yield, of an optically thick, homogeneous, turbid medium from a surface measurement of fluorescence and from knowledge of medium optical properties. The measured fluorescence signal is related to the intrinsic fluorescence coefficient by an optical property dependent path–length factor. A simple expression was developed for the path–length factor, which characterizes the penetration of excitation light and the escape of fluorescence from the medium. Experiments with fluorescent tissue phantoms demonstrated that intrinsic fluorescence line shape could be recovered and that fluorophore concentration could be estimated within 615%, over a wide range of optical properties.

Original languageEnglish (US)
Pages (from-to)1780-1792
Number of pages13
JournalApplied Optics
Volume35
Issue number10
DOIs
StatePublished - Apr 1 1996
Externally publishedYes

Keywords

  • Diagnostics
  • Fluorescence
  • Light transport
  • Photodynamic therapy
  • Photosensitizer

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Engineering (miscellaneous)
  • Electrical and Electronic Engineering

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