Fluorescence spectroscopy of tissue: Recovery of intrinsic fluorescence from measured fluorescence

Craig M. Gardner, Steven Jacques, Ashley J. Welch

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

We present a method for recovering the intrinsic fluorescence coefficient, defined as the product of the fluorophore absorption coefficient and the fluorescence energy yield, of an optically thick, homogeneous, turbid medium from a surface measurement of fluorescence and from knowledge of medium optical properties. The measured fluorescence signal is related to the intrinsic fluorescence coefficient by an optical property dependent path-length factor. A simple expression was developed for the path-length factor, which characterizes the penetration of excitation light and the escape of fluorescence from the medium. Experiments with fluorescent tissue phantoms demonstrated that intrinsic fluorescence line shape could be recovered and that fluorophore concentration could be estimated within ±15%, over a wide range of optical properties.

Original languageEnglish (US)
Pages (from-to)1780-1792
Number of pages13
JournalApplied Optics
Volume35
Issue number10
StatePublished - Apr 1 1996
Externally publishedYes

Fingerprint

Fluorescence spectroscopy
Fluorescence
recovery
Tissue
Recovery
fluorescence
spectroscopy
Fluorophores
Optical properties
optical properties
Surface measurement
coefficients
escape
line shape
absorptivity
penetration
products
excitation

Keywords

  • Diagnostics
  • Fluorescence
  • Light transport
  • Photodynamic therapy
  • Photosensitizer

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Gardner, C. M., Jacques, S., & Welch, A. J. (1996). Fluorescence spectroscopy of tissue: Recovery of intrinsic fluorescence from measured fluorescence. Applied Optics, 35(10), 1780-1792.

Fluorescence spectroscopy of tissue : Recovery of intrinsic fluorescence from measured fluorescence. / Gardner, Craig M.; Jacques, Steven; Welch, Ashley J.

In: Applied Optics, Vol. 35, No. 10, 01.04.1996, p. 1780-1792.

Research output: Contribution to journalArticle

Gardner, CM, Jacques, S & Welch, AJ 1996, 'Fluorescence spectroscopy of tissue: Recovery of intrinsic fluorescence from measured fluorescence', Applied Optics, vol. 35, no. 10, pp. 1780-1792.
Gardner, Craig M. ; Jacques, Steven ; Welch, Ashley J. / Fluorescence spectroscopy of tissue : Recovery of intrinsic fluorescence from measured fluorescence. In: Applied Optics. 1996 ; Vol. 35, No. 10. pp. 1780-1792.
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