Fluorescence photobleaching recovery measurements reveal differences in envelopment of sindbis and vesicular stomatitis viruses

David Johnson, Milton J. Schlesinger, Elliot L. Elson

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Fluorescence photobleaching recovery (FPR) measurements of virus glycoproteins on the surfaces of cells infected with vesicular stomatitis virus (VSV) and Sindbis virus showed that the VSV glycoprotein (G) remained mobile throughout the infectious cycle, whereas Sindbis virus glycoproteins (E1, E2) were partially mobile early after infection and immobile at later times when greater amounts of these proteins were on the cell surface. A highly mobile fraction of Sindbis virus glycoproteins was detected throughout the replication cycle of a temperature-sensitive mutant unable to form virus particles. Thus immobilization of E1 and E2 was the result of increasing surface glycoprotein concentrations and virus budding. Together with other data, which included the detection of E1 and E2 in particles as soon as these proteins were transported to the cell surface, the FPR results suggest that Sindbis virus assembly initiates on intracellular vesicles, where glycoproteins aggregate and bind nucleocapsids. In contrast, our FPR data on VSV support a model previously suggested by others, in which a small fraction of cell-surface G is immobilized into budding sites formed by interactions with virus matrix and nucleoproteins. FPR measurements also provide direct evidence for strong interactions between E1 and E2, as well as between E1 and PE2, the precursor form of E2.

Original languageEnglish (US)
Pages (from-to)423-431
Number of pages9
JournalCell
Volume23
Issue number2
DOIs
StatePublished - 1981
Externally publishedYes

Fingerprint

Fluorescence Recovery After Photobleaching
Photobleaching
Vesicular Stomatitis
Viruses
Sindbis Virus
Fluorescence
Recovery
Glycoproteins
Membrane Glycoproteins
Virus Release
Virus Assembly
Nucleocapsid
Gastrin-Secreting Cells
Nucleoproteins
Immobilization
Virion
Proteins
Temperature
Infection

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Fluorescence photobleaching recovery measurements reveal differences in envelopment of sindbis and vesicular stomatitis viruses. / Johnson, David; Schlesinger, Milton J.; Elson, Elliot L.

In: Cell, Vol. 23, No. 2, 1981, p. 423-431.

Research output: Contribution to journalArticle

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