Fluorescence in situ hybridization with human chromosome-specific libraries: Detection of trisomy 21 and translocations of chromosome 4

D. Pinkel; J. Landegent; C. Collins; J. Fuscoe; R. Segraves; J. Lucas; J. Gray

doi:10.1073/pnas.85.23.9138

Fluorescence in situ hybridization with human chromosome-specific libraries: Detection of trisomy 21 and translocations of chromosome 4

D. Pinkel, J. Landegent, C. Collins, J. Fuscoe, R. Segraves, J. Lucas, J. Gray

Research output: Contribution to journal › Article › peer-review

1257 Scopus citations

Abstract

Chromosomes can be specifically stained in metaphase spreads and interphase nuclei by in situ hybridization with entire chromosome-specific DNA libraries. Unlabeled human genomic DNA is used to inhibit the hybridization of sequences in the library that bind to multiple chromosomes. The target chromosome can be made at least 20 times brighter per unit length than the others. Trisomy 21 and translocations involving chromosome 4 can be detected in metaphase spreads and interphase nuclei by using this technique.

Original language	English (US)
Pages (from-to)	9138-9142
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	85
Issue number	23
DOIs	https://doi.org/10.1073/pnas.85.23.9138
State	Published - 1988
Externally published	Yes

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Pinkel, D., Landegent, J., Collins, C., Fuscoe, J., Segraves, R., Lucas, J., & Gray, J. (1988). Fluorescence in situ hybridization with human chromosome-specific libraries: Detection of trisomy 21 and translocations of chromosome 4. Proceedings of the National Academy of Sciences of the United States of America, 85(23), 9138-9142. https://doi.org/10.1073/pnas.85.23.9138

Fluorescence in situ hybridization with human chromosome-specific libraries: Detection of trisomy 21 and translocations of chromosome 4. / Pinkel, D.; Landegent, J.; Collins, C. et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, No. 23, 1988, p. 9138-9142.

Research output: Contribution to journal › Article › peer-review

Pinkel, D, Landegent, J, Collins, C, Fuscoe, J, Segraves, R, Lucas, J & Gray, J 1988, 'Fluorescence in situ hybridization with human chromosome-specific libraries: Detection of trisomy 21 and translocations of chromosome 4', Proceedings of the National Academy of Sciences of the United States of America, vol. 85, no. 23, pp. 9138-9142. https://doi.org/10.1073/pnas.85.23.9138

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Fluorescence in situ hybridization with human chromosome-specific libraries: Detection of trisomy 21 and translocations of chromosome 4

Abstract

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