Fluorescence and bioluminescence procedures for functional proteomics

Anke Prinz, Gregor Reither, Mandy Diskar, Carsten Schultz

Research output: Contribution to journalReview article

25 Scopus citations

Abstract

This review aims to provide an overview of current optical procedures used in functional proteomics, investigating protein localization, protein-protein interaction, intracellular signaling events, and second messenger generation in living cells. Reporter assays using proteins tagged with fluorescent or bioluminescent moieties are discussed. Recently, intracellular biosensor assays, flow cytometry-based techniques (fluorescent cell barcoding), as well as transfected cell microarray assays involving RNA interference coupled with automated imaging were introduced and have been adopted as screening platforms for annotating small molecules, investigating signaling events, or in phenotype analysis. These novel methodological advances include improved image acquisition and processing techniques and help linking in vitro observations to in vivo processes. In addition, the acquired data are increasingly quantitative in nature and will therefore pave the way for modeling of signaling cascades and other complex cellular events, an important step toward systems biology.

Original languageEnglish (US)
Pages (from-to)1179-1196
Number of pages18
JournalProteomics
Volume8
Issue number6
DOIs
StatePublished - Mar 1 2008

Keywords

  • Bioluminescence
  • Fluorescence
  • Imaging
  • Protein-protein interaction
  • Resonance energy transfer

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'Fluorescence and bioluminescence procedures for functional proteomics'. Together they form a unique fingerprint.

  • Cite this