Fluorescence and bioluminescence procedures for functional proteomics

Anke Prinz, Gregor Reither, Mandy Diskar, Carsten Schultz

Research output: Contribution to journalReview article

24 Citations (Scopus)

Abstract

This review aims to provide an overview of current optical procedures used in functional proteomics, investigating protein localization, protein-protein interaction, intracellular signaling events, and second messenger generation in living cells. Reporter assays using proteins tagged with fluorescent or bioluminescent moieties are discussed. Recently, intracellular biosensor assays, flow cytometry-based techniques (fluorescent cell barcoding), as well as transfected cell microarray assays involving RNA interference coupled with automated imaging were introduced and have been adopted as screening platforms for annotating small molecules, investigating signaling events, or in phenotype analysis. These novel methodological advances include improved image acquisition and processing techniques and help linking in vitro observations to in vivo processes. In addition, the acquired data are increasingly quantitative in nature and will therefore pave the way for modeling of signaling cascades and other complex cellular events, an important step toward systems biology.

Original languageEnglish (US)
Pages (from-to)1179-1196
Number of pages18
JournalProteomics
Volume8
Issue number6
DOIs
StatePublished - Mar 2008
Externally publishedYes

Fingerprint

Bioluminescence
Proteomics
Fluorescence
Assays
Intracellular Signaling Peptides and Proteins
Proteins
Systems Biology
Biosensing Techniques
Second Messenger Systems
RNA Interference
Flow cytometry
Image acquisition
Flow Cytometry
Microarrays
Biosensors
Phenotype
Screening
Image processing
Cells
RNA

Keywords

  • Bioluminescence
  • Fluorescence
  • Imaging
  • Protein-protein interaction
  • Resonance energy transfer

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Fluorescence and bioluminescence procedures for functional proteomics. / Prinz, Anke; Reither, Gregor; Diskar, Mandy; Schultz, Carsten.

In: Proteomics, Vol. 8, No. 6, 03.2008, p. 1179-1196.

Research output: Contribution to journalReview article

Prinz, Anke ; Reither, Gregor ; Diskar, Mandy ; Schultz, Carsten. / Fluorescence and bioluminescence procedures for functional proteomics. In: Proteomics. 2008 ; Vol. 8, No. 6. pp. 1179-1196.
@article{95dae5d9ec7443d2b2720d09dda3bb49,
title = "Fluorescence and bioluminescence procedures for functional proteomics",
abstract = "This review aims to provide an overview of current optical procedures used in functional proteomics, investigating protein localization, protein-protein interaction, intracellular signaling events, and second messenger generation in living cells. Reporter assays using proteins tagged with fluorescent or bioluminescent moieties are discussed. Recently, intracellular biosensor assays, flow cytometry-based techniques (fluorescent cell barcoding), as well as transfected cell microarray assays involving RNA interference coupled with automated imaging were introduced and have been adopted as screening platforms for annotating small molecules, investigating signaling events, or in phenotype analysis. These novel methodological advances include improved image acquisition and processing techniques and help linking in vitro observations to in vivo processes. In addition, the acquired data are increasingly quantitative in nature and will therefore pave the way for modeling of signaling cascades and other complex cellular events, an important step toward systems biology.",
keywords = "Bioluminescence, Fluorescence, Imaging, Protein-protein interaction, Resonance energy transfer",
author = "Anke Prinz and Gregor Reither and Mandy Diskar and Carsten Schultz",
year = "2008",
month = "3",
doi = "10.1002/pmic.200700802",
language = "English (US)",
volume = "8",
pages = "1179--1196",
journal = "Proteomics",
issn = "1615-9853",
publisher = "Wiley-VCH Verlag",
number = "6",

}

TY - JOUR

T1 - Fluorescence and bioluminescence procedures for functional proteomics

AU - Prinz, Anke

AU - Reither, Gregor

AU - Diskar, Mandy

AU - Schultz, Carsten

PY - 2008/3

Y1 - 2008/3

N2 - This review aims to provide an overview of current optical procedures used in functional proteomics, investigating protein localization, protein-protein interaction, intracellular signaling events, and second messenger generation in living cells. Reporter assays using proteins tagged with fluorescent or bioluminescent moieties are discussed. Recently, intracellular biosensor assays, flow cytometry-based techniques (fluorescent cell barcoding), as well as transfected cell microarray assays involving RNA interference coupled with automated imaging were introduced and have been adopted as screening platforms for annotating small molecules, investigating signaling events, or in phenotype analysis. These novel methodological advances include improved image acquisition and processing techniques and help linking in vitro observations to in vivo processes. In addition, the acquired data are increasingly quantitative in nature and will therefore pave the way for modeling of signaling cascades and other complex cellular events, an important step toward systems biology.

AB - This review aims to provide an overview of current optical procedures used in functional proteomics, investigating protein localization, protein-protein interaction, intracellular signaling events, and second messenger generation in living cells. Reporter assays using proteins tagged with fluorescent or bioluminescent moieties are discussed. Recently, intracellular biosensor assays, flow cytometry-based techniques (fluorescent cell barcoding), as well as transfected cell microarray assays involving RNA interference coupled with automated imaging were introduced and have been adopted as screening platforms for annotating small molecules, investigating signaling events, or in phenotype analysis. These novel methodological advances include improved image acquisition and processing techniques and help linking in vitro observations to in vivo processes. In addition, the acquired data are increasingly quantitative in nature and will therefore pave the way for modeling of signaling cascades and other complex cellular events, an important step toward systems biology.

KW - Bioluminescence

KW - Fluorescence

KW - Imaging

KW - Protein-protein interaction

KW - Resonance energy transfer

UR - http://www.scopus.com/inward/record.url?scp=41549110645&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=41549110645&partnerID=8YFLogxK

U2 - 10.1002/pmic.200700802

DO - 10.1002/pmic.200700802

M3 - Review article

C2 - 18283669

AN - SCOPUS:41549110645

VL - 8

SP - 1179

EP - 1196

JO - Proteomics

JF - Proteomics

SN - 1615-9853

IS - 6

ER -